Unveiling the potential of Bacillus cereus for the biodegradation of antibiotic metronidazole in aqueous solutions

IF 5.5 3区工程技术 Q1 ENGINEERING, CHEMICAL

Journal of the Taiwan Institute of Chemical Engineers Pub Date : 2025-05-16 DOI:10.1016/j.jtice.2025.106192

Mai Lien Tran , Yu-Chieh Hsieh , John Chi-Wei Lan , Ruey-Shin Juang

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引用次数: 0

Abstract

Background

:Wastewaters discharged from pastures, hospitals, and some factories often contain trace amounts of various antibiotics. If these antibiotics are not properly treated, they will cause bacterial resistance and have a new impact on human health.

Methods

:In this study, bacteria were first adopted for solid-state antibiotic culture tests, while contaminating bacteria identified to be Bacillus cereus (B. cereus) were selected and cultured. After culture in liquid medium, different concentrations of metronidazole (MNZ) were added for further degradation tests.

Significant Findings

:The results revealed that MNZ could be degraded although MNZ has an inhibitory effect on B. cereus growth. The Haldane model was used to analyze cell growth kinetics. The maximum specific growth rate μ_max, affinity constant K_S, and inhibition constant K_I were calculated to be 0.0158 h^-1, 29.7 mg/L, and 267.0 mg/L, respectively. The GC–MS analysis showed that only acetic acid was detected during the biodegradation of MNZ, suggesting possible MNZ degradation pathways.

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揭示蜡样芽孢杆菌在水溶液中生物降解抗生素甲硝唑的潜力

背景：牧场、医院和一些工厂排放的废水通常含有微量的各种抗生素。这些抗生素如果处理不当，就会引起细菌耐药性，对人类健康产生新的影响。方法：本研究首先采用细菌进行固体抗生素培养试验，同时选取经鉴定为蜡样芽孢杆菌（B. cereus）的污染菌进行培养。在液体培养基中培养后，加入不同浓度的甲硝唑（MNZ）进行进一步的降解试验。重要发现：MNZ虽然对蜡样芽孢杆菌生长有抑制作用，但可以降解。采用Haldane模型分析细胞生长动力学。最大比生长速率μmax、亲和常数KS和抑制常数KI分别为0.0158 h-1、29.7 mg/L和267.0 mg/L。GC-MS分析表明，MNZ生物降解过程中只检测到乙酸，提示MNZ可能的降解途径。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Taiwan Institute of Chemical Engineers 工程技术-工程：化工

CiteScore

9.10

自引率

14.00%

发文量

362

审稿时长

35 days

期刊介绍： Journal of the Taiwan Institute of Chemical Engineers (formerly known as Journal of the Chinese Institute of Chemical Engineers) publishes original works, from fundamental principles to practical applications, in the broad field of chemical engineering with special focus on three aspects: Chemical and Biomolecular Science and Technology, Energy and Environmental Science and Technology, and Materials Science and Technology. Authors should choose for their manuscript an appropriate aspect section and a few related classifications when submitting to the journal online.