One-pot RPA-CRISPR/Cas12a integrated dual-mode electrochemical lateral flow strip for ultrasensitive and precise detection of Salmonella

IF 10.7 1区生物学 Q1 BIOPHYSICS

Biosensors and Bioelectronics Pub Date : 2025-05-01 DOI:10.1016/j.bios.2025.117529

Qi Chen , Hang Wang , Haoyang Xu , Yubo Peng , Bangben Yao , Zhaoran Chen , Jiening Yang , Samuel Adeloju , Wei Chen

引用次数: 0

Abstract

Rapid and accurate screening of pathogenic contamination is essential for timely intervention and infection prevention. In this work, one-pot RPA-CRISPR/Cas12a strategy combined with an innovative electrochemical lateral flow strip (OPRCC-eLFS) was presented for ultrasensitive and precise detection of Salmonella. Highly sensitive dual-mode detection of Salmonella in various samples has been simultaneously achieved with electrochemical detection limit of 3.84 CFU/mL and visual detection limit of 384 CFU/mL, respectively, with improved detection efficiency and prevention of uncapping-related aerosol-contamination. This dual-mode biosensing platform demonstrates exceptional stability, remarkable sensitivity, and robust on-site quantification capability, emphasizing its potential in food safety monitoring and disease prevention.

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一锅RPA-CRISPR/Cas12a集成双模电化学横向流动条带，用于沙门氏菌的超灵敏和精确检测

快速准确的病原污染筛查对于及时干预和预防感染至关重要。本研究采用一锅RPA-CRISPR/Cas12a策略结合新型电化学横向流动条带（OPRCC-eLFS）对沙门氏菌进行了超灵敏、精确的检测。同时实现了多种样品中沙门氏菌的高灵敏度双模检测，电化学检出限为3.84 CFU/mL，目测检出限为384 CFU/mL，提高了检测效率，防止了揭盖引起的气溶胶污染。该双模生物传感平台具有优异的稳定性、卓越的灵敏度和强大的现场定量能力，强调了其在食品安全监测和疾病预防方面的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biosensors and Bioelectronics 工程技术-电化学

CiteScore

20.80

自引率

7.10%

发文量

1006

审稿时长

29 days

期刊介绍： Biosensors & Bioelectronics, along with its open access companion journal Biosensors & Bioelectronics: X, is the leading international publication in the field of biosensors and bioelectronics. It covers research, design, development, and application of biosensors, which are analytical devices incorporating biological materials with physicochemical transducers. These devices, including sensors, DNA chips, electronic noses, and lab-on-a-chip, produce digital signals proportional to specific analytes. Examples include immunosensors and enzyme-based biosensors, applied in various fields such as medicine, environmental monitoring, and food industry. The journal also focuses on molecular and supramolecular structures for enhancing device performance.