Two-electrode electrochemical scans vs. three-electrode scans in microbiologically influenced corrosion detection with various counter electrode/pseudo-reference electrode materials

IF 4.8 2区化学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioelectrochemistry Pub Date : 2025-05-13 DOI:10.1016/j.bioelechem.2025.109002

Lingjun Xu , Adnan Khan , Di Wang , Pruch Kijkla , Sith Kumseranee , Suchada Punpruk , Sarah A. Aqeel , Tingyue Gu

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引用次数: 0

Abstract

Electrochemical measurements are popular in lab microbiologically influenced corrosion (MIC) studies. A classical 3-electrode (3E) setup is not suitable for biofilm/MIC test kits use because the reference electrode (RE) is fragile. For biofilm/MIC test kit applications, the counter electrode (CE) is used as CE and also pseudo-RE (p-RE) to create a 2-electrode (2E) setup. This work compared 2E and 3E scan results in a 450 mL glass cell containing sulfate reducing bacteria (SRB) with platinum and graphite serving as CE materials. In both SRB incubation and injection tests, 2E scan results turned out to be as reliable as 3E scan results in reflecting corrosion rates. The chemical injection test with platinum CE showed that after riboflavin, magnetite nanoparticles and tetrakis hydroxymethyl phosphonium sulfate biocide injections, the polarization resistance of X65 WEs shifted by −8.8 %, −6.2 % and 11 %, respectively in 3E scan, and − 6.6 %, −7.1 % and 8.3 %, respectively in 2E scan. The 2E and 3E scan results in a 10 mL miniature glass cell also correctly reflected MIC information indicating SRB biofilm maturity at 3 d. This study found that 316L SS, Cr, Ni and Mo can be potentially used as additional choices for CE/p-RE materials in biofilm/MIC test kit.

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两电极电化学扫描与三电极扫描在各种对电极/伪参比电极材料的微生物影响腐蚀检测

电化学测量在实验室微生物影响腐蚀（MIC）研究中很受欢迎。经典的3电极（3E）设置不适合用于生物膜/MIC测试试剂盒，因为参比电极（RE）易碎。对于生物膜/MIC测试试剂盒应用，对电极（CE）用作CE，也使用伪re （p-RE）来创建2电极（2E）设置。在450 mL含硫酸盐还原菌（SRB）的玻璃池中，用铂和石墨作为CE材料，比较了2E和3E扫描结果。在SRB孵育和注入试验中，2E扫描结果在反映腐蚀速率方面与3E扫描结果一样可靠。铂CE化学注射试验表明，核黄素、磁铁矿纳米粒子和四甲基硫酸氢磷杀生剂注射后，X65 WEs的极化电阻在3E扫描中分别变化了−8.8%、−6.2%和11%，在2E扫描中分别变化了−6.6%、−7.1%和8.3%。10 mL微型玻璃细胞的2E和3E扫描结果也正确反映了表明SRB生物膜在3 d时成熟的MIC信息。本研究发现，316L SS、Cr、Ni和Mo可以潜在地用作生物膜/MIC测试试剂盒中CE/p-RE材料的额外选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioelectrochemistry 生物-电化学

CiteScore

9.10

自引率

6.00%

发文量

238

审稿时长

38 days

期刊介绍： An International Journal Devoted to Electrochemical Aspects of Biology and Biological Aspects of Electrochemistry Bioelectrochemistry is an international journal devoted to electrochemical principles in biology and biological aspects of electrochemistry. It publishes experimental and theoretical papers dealing with the electrochemical aspects of: • Electrified interfaces (electric double layers, adsorption, electron transfer, protein electrochemistry, basic principles of biosensors, biosensor interfaces and bio-nanosensor design and construction. • Electric and magnetic field effects (field-dependent processes, field interactions with molecules, intramolecular field effects, sensory systems for electric and magnetic fields, molecular and cellular mechanisms) • Bioenergetics and signal transduction (energy conversion, photosynthetic and visual membranes) • Biomembranes and model membranes (thermodynamics and mechanics, membrane transport, electroporation, fusion and insertion) • Electrochemical applications in medicine and biotechnology (drug delivery and gene transfer to cells and tissues, iontophoresis, skin electroporation, injury and repair). • Organization and use of arrays in-vitro and in-vivo, including as part of feedback control. • Electrochemical interrogation of biofilms as generated by microorganisms and tissue reaction associated with medical implants.