Surface-enhanced Raman spectroscopy based on 2,3-naphthalenedicarboxaldehyde derivative reagent for highly sensitive detection of cysteine and homocysteine

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2025-05-14 DOI:10.1016/j.saa.2025.126395

Qianqian Zhang, Shengping Han, Guican Deng, Xianzai Yan, Jiaxin Hong, Luying Duan, Guoqiang Wu, Yanping Hong, Lili Yu, Chunrong Wang

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引用次数: 0

Abstract

The study presents a simple, efficient, and sensitive methodology which combines condensation reaction with surface-enhanced Raman spectroscopy (SERS) to detect cysteine (Cys) and homocysteine (Hcy). Experimental conditions which include reaction temperature, time, and buffer pH can be used to influence the derivatization reaction of 2,3-dinaphthaldehyde (NDA) with Cys and Hcy. Quantitative analysis was conducted on the characteristic peaks of the products of Cys (1377 cm⁻¹) and Hcy (665 cm⁻¹) after reacting with NDA. The intensity of characteristic Raman peaks is linearly related to the concentrations of Cys and Hcy (10⁻¹²–10⁻⁴ mol/L), with a correlation coefficient R² greater than 0.99. This method combines SERS and derivatization reaction, has significant application potential for detecting Cys and Hcy in biological media because to its excellent sensitivity and simplified use.

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基于2,3-萘二甲酸衍生物试剂的表面增强拉曼光谱高灵敏度检测半胱氨酸和同型半胱氨酸

本研究提出了一种结合缩合反应和表面增强拉曼光谱（SERS）检测半胱氨酸（Cys）和同型半胱氨酸（Hcy）的简单、高效、灵敏的方法。影响2,3-二萘醛（NDA）与Cys和Hcy衍生化反应的实验条件包括反应温度、时间和缓冲液pH。对与NDA反应后产物Cys （1377 cm−1）和Hcy （665 cm−1）的特征峰进行定量分析。特征拉曼峰强度与Cys和Hcy浓度（10−12 ~ 10−4 mol/L）呈线性相关，相关系数R2 > 0.99。该方法结合了SERS和衍生化反应，灵敏度高，使用简便，在生物介质中检测Cys和Hcy具有重要的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.