Transcription factor OsNAC29a confers drought tolerance through the ABA pathway in rice

IF 6.1 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2025-05-07 DOI:10.1016/j.plaphy.2025.109989

Jia Lu , Weiting Wang , Siqi Yang, Liwen Shi, Fangyuan Song, Yulei Tan, Xiaocui Wu, Baocun Zhao

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Abstract

The plant-specific NAC transcription factor family plays a crucial role in mediating responses to abiotic stress, but the functions of many NAC genes remain poorly characterized. The rice OsNAC29a gene is induced by PEG and abscisic acid (ABA). OsNAC29a exhibits transactivation activity and the region of 248–315 amino acids at its C-terminus is essential for its activation. Over-expression of OsNAC29a enhances drought resistance and ABA sensitivity in transgenic rice. OsNAC29a over-expression modulates physiological indicators related to stress resistance, while RNAi-mediated down-regulation of OsNAC29a results in opposite phenotypes and physiological changes. Under drought conditions, OsNAC29a over-expression significantly up-regulates stress-related genes such as OsP5CS1, OsSRO1c, OsPOD1, OsLEA3, and OsRab16C. Interestingly, OsPOD1 gene expression increases in OsNAC29a over-expression rice under both normal and drought stress conditions, leading to significantly enhanced peroxidase activity. Further research reveals that OsNAC29a binds to the OsPOD1 promoter to drive its expression. Additionally, OsSAPK2 which is a key component of the ABA-dependent drought-tolerance pathway interacts physically with OsNAC29a and enhances its transcriptional activation activity. Collectively, acting as a positive regulator of drought tolerance, OsNAC29a regulates drought resistance in rice by directly or indirectly modulating stress-responsive genes.

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转录因子OsNAC29a通过ABA通路赋予水稻抗旱性

植物特异性NAC转录因子家族在介导非生物胁迫应答中起着至关重要的作用，但许多NAC基因的功能仍不清楚。水稻OsNAC29a基因是由PEG和脱落酸（ABA）诱导的。OsNAC29a具有活化活性，其c端有248-315个氨基酸，是其活化所必需的区域。OsNAC29a的过表达增强了转基因水稻的抗旱性和ABA敏感性。OsNAC29a过表达调节与抗逆性相关的生理指标，而rnai介导的OsNAC29a下调则导致相反的表型和生理变化。干旱条件下，OsNAC29a过表达显著上调胁迫相关基因OsP5CS1、OsSRO1c、OsPOD1、OsLEA3和OsRab16C。有趣的是，OsNAC29a过表达水稻在正常和干旱胁迫条件下，OsPOD1基因表达增加，导致过氧化物酶活性显著增强。进一步研究表明，OsNAC29a结合OsPOD1启动子驱动其表达。此外，OsSAPK2是aba依赖性抗旱途径的关键组成部分，与OsNAC29a发生物理相互作用，增强其转录激活活性。综上所述，OsNAC29a作为水稻抗旱性的正向调节因子，通过直接或间接调节胁迫应答基因来调节水稻的抗旱性。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.