Reactive species dynamics and bactericidal mechanisms in cold atmospheric pressure plasma with gas admixtures

IF 3.8 2区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

Vacuum Pub Date : 2025-05-08 DOI:10.1016/j.vacuum.2025.114393

Vishakha Bende , Vandan Nagar , P.S.N.S.R. Srikar , Reetesh K. Gangwar , Devendra Bhale , R.L. Bhardwaj , Rajib Kar

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Abstract

This study investigates reactive species dynamics in cold atmospheric pressure plasma (CAPP) and bacterial inactivation pathways in Escherichia coli (E.coli) and Staphylococcus aureus (S.aureus) using a Tesla coil-based radiofrequency (10 MHz) plasma jet with four gas compositions: Ar, Ar-O₂, Ar-N₂, and Ar-dry air (3:1) at a 10 mm treatment distance. Optical emission spectroscopy identified •OH and OI, while absorption and fluorescence spectroscopy quantified H₂O₂ and •OH. UV radiation and O₃ were also measured. Electron temperature (T_e) and density (n_e) were determined via collisional-radiative modeling and Stark broadening analysis. Pure Ar plasma achieved complete inactivation, whereas gas admixtures, with lower T_e and n_e, reduced reactive species production. Despite its thicker peptidoglycan layer, S.aureus was more susceptible than E.coli, likely due to E.coli's outer membrane limiting radical penetration. Ar-N₂ caused a 3-log reduction in S.aureus but only 2-log in E.coli, while Ar-O₂ and Ar-dry air had minimal effects on E.coli but reduced S.aureus by 1-log and 2-log, respectively. H₂O₂ and UV played key roles in oxidative stress and DNA damage, with H₂O₂ facilitating intracellular •OH generation via Fenton reactions. Pure Ar plasma demonstrated strong bactericidal efficiency at 10 mm, driven by long-lived species (H₂O₂, UV) essential for contactless treatment.

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含气体外加剂的冷大气压等离子体中的反应态动力学和杀菌机制

本研究利用基于特斯拉线圈的射频（10 MHz）等离子体射流，在10 mm的处理距离上，使用Ar、Ar- o2、Ar- n2和Ar干空气（3:1）四种气体成分，研究冷大气压等离子体（CAPP）中的反应物种动力学和细菌失活途径。光学发射光谱鉴定了•OH和OI，而吸收光谱和荧光光谱定量了H2O2和•OH。同时测量了紫外线辐射和臭氧。通过碰撞辐射模型和Stark展宽分析确定了电子温度（Te）和密度（ne）。纯Ar等离子体实现了完全失活，而含有较低Te和ne的气体外加剂则减少了反应物质的产生。尽管金黄色葡萄球菌的肽聚糖层较厚，但它比大肠杆菌更敏感，这可能是由于大肠杆菌的外膜限制了自由基的渗透。Ar-N2对金黄色葡萄球菌的抑制作用为3对数，对大肠杆菌的抑制作用为2对数；Ar-O2和ar -干燥空气对大肠杆菌的抑制作用最小，但对金黄色葡萄球菌的抑制作用分别为1对数和2对数。H2O2和UV在氧化应激和DNA损伤中起关键作用，H2O2通过Fenton反应促进细胞内•OH生成。纯Ar等离子体在10毫米处显示出很强的杀菌效率，这是由非接触处理所必需的长寿命物种（H2O2， UV）驱动的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Vacuum 工程技术-材料科学：综合

CiteScore

6.80

自引率

17.50%

发文量

审稿时长

34 days

期刊介绍： Vacuum is an international rapid publications journal with a focus on short communication. All papers are peer-reviewed, with the review process for short communication geared towards very fast turnaround times. The journal also published full research papers, thematic issues and selected papers from leading conferences. A report in Vacuum should represent a major advance in an area that involves a controlled environment at pressures of one atmosphere or below. The scope of the journal includes: 1. Vacuum; original developments in vacuum pumping and instrumentation, vacuum measurement, vacuum gas dynamics, gas-surface interactions, surface treatment for UHV applications and low outgassing, vacuum melting, sintering, and vacuum metrology. Technology and solutions for large-scale facilities (e.g., particle accelerators and fusion devices). New instrumentation ( e.g., detectors and electron microscopes). 2. Plasma science; advances in PVD, CVD, plasma-assisted CVD, ion sources, deposition processes and analysis. 3. Surface science; surface engineering, surface chemistry, surface analysis, crystal growth, ion-surface interactions and etching, nanometer-scale processing, surface modification. 4. Materials science; novel functional or structural materials. Metals, ceramics, and polymers. Experiments, simulations, and modelling for understanding structure-property relationships. Thin films and coatings. Nanostructures and ion implantation.