Effects of high fat diet on blood lipids and liver enzymes in murine model: the systemic and experimental study

Q2 Medicine

Obesity Medicine Pub Date : 2025-05-01 DOI:10.1016/j.obmed.2025.100614

Thuy-Duong Vu , Anh-Dao Ngo , Son-Tung Nguyen , Yen-Nhi Khuong , Van-Bao Phan , Tien-Dat Pham , Duc-Anh Le , Thanh-Thuy To , Chia-Ching Wu , Dinh-Toi Chu

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引用次数: 0

Abstract

Background

A high fat diet is associated various metabolic disorders, including fatty liver disease and dyslipidemia. This work is aimed to examine effects of excess lard usage from diet on blood lipids and liver enzymes in murine models in the aspects of genetics and food types.

Methods

A systematic review was conducted to form a general picture of the effects of high fat diet (HFD) on blood lipid and liver enzyme profiles of mice and compare these indices between study designs. At the same time, 24 Swiss albino mice were included in an experimental study, two groups with different diets (HFD and standard diet - STD) in 18 weeks to examine serum levels of liver enzymes and blood lipids.

Results

Our results in the systematic review indicate that HFD from lard increased TG, TC, LDL-C, ALT indexes in the majority of studies. C57BL/6 strain was used the most as well as being seen as the best results in these studies. In addition, the most popular types of HFD used in these results were HFD 60 % and HFD 45 %. They also showed the most appropriate results, in which those related to HFD 60 % had lower dispersion than those of HFD 40 %. The change in blood lipids and serum liver enzymes due to HFD from lard also varied among studies using the same strain as well as different strains, especially that of HDL-C and AST. Our experiment on Swiss mice confirmed these results. After 18 weeks eating HFD, mice gained weight and accumulated white fat. All four indices of blood lipids including TC, TG, HDL-C and LDL-C of HFD group were significantly higher than STD group, with 15.90 %, 28.49 %, 20.12 % and 41.79 %, respectively. Although AST level did not show a difference between two groups, HFD leads to a 1.29-fold-increase in ALT level in mice.

Conclusion

In conclusion, both our systematic and experimental results indicate that HFD can increase blood lipids and liver enzymes in mice, and systematic analysis suggests that dyslipidemia and detrimental effects on the liver by HFD are depending on the animal genetics and types of diet.

查看原文本刊更多论文

高脂饮食对小鼠血脂及肝酶的影响：系统与实验研究

背景：高脂肪饮食与多种代谢紊乱有关，包括脂肪肝和血脂异常。本研究旨在从遗传学和食物类型方面研究饮食中过量使用猪油对小鼠血脂和肝酶的影响。方法对高脂饮食对小鼠血脂和肝酶谱的影响进行系统评价，并在不同的研究设计之间进行比较。同时，24只瑞士白化病小鼠被纳入一项实验研究，两组在18周内采用不同的饮食（HFD和标准饮食- STD）来检测血清肝酶和血脂水平。结果我们的系统综述结果表明，在大多数研究中，来自猪油的HFD增加了TG、TC、LDL-C、ALT指标。C57BL/6菌株在这些研究中使用最多，效果最好。此外，在这些结果中，最常用的HFD类型是HFD 60%和HFD 45%。他们还显示了最合适的结果，即与HFD 60%相关的患者的弥散度低于HFD 40%相关的患者。来自猪油的HFD引起的血脂和血清肝酶的变化在使用同一菌株和不同菌株的研究中也有所不同，特别是HDL-C和AST。我们在瑞士小鼠身上的实验证实了这些结果。食用HFD 18周后，小鼠体重增加，白色脂肪堆积。HFD组的TC、TG、HDL-C、LDL-C 4项血脂指标均显著高于STD组，分别为15.90%、28.49%、20.12%、41.79%。虽然两组间谷丙转氨酶水平没有差异，但HFD导致小鼠谷丙转氨酶水平升高1.29倍。综上所述，我们的系统和实验结果都表明，HFD可以增加小鼠的血脂和肝酶，系统分析表明，HFD对肝脏的血脂异常和有害影响与动物的遗传和饮食类型有关。

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来源期刊

Obesity Medicine Medicine-Public Health, Environmental and Occupational Health

CiteScore

5.50

自引率

0.00%

发文量

审稿时长

40 days

期刊介绍： The official journal of the Shanghai Diabetes Institute Obesity is a disease of increasing global prevalence with serious effects on both the individual and society. Obesity Medicine focusses on health and disease, relating to the very broad spectrum of research in and impacting on humans. It is an interdisciplinary journal that addresses mechanisms of disease, epidemiology and co-morbidities. Obesity Medicine encompasses medical, societal, socioeconomic as well as preventive aspects of obesity and is aimed at researchers, practitioners and educators alike.