Blinking Nanoparticles as Super-Resolution Bioimaging Probes: A Review

IF 5.5 2区材料科学 Q2 MATERIALS SCIENCE, MULTIDISCIPLINARY

ACS Applied Nano Materials Pub Date : 2025-05-02 DOI:10.1021/acsanm.5c0079110.1021/acsanm.5c00791

Debiprasad Roy, Reeddhi Ray, Jayanta Dolai* and Nikhil R. Jana*,

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Abstract

Super-resolution microscopy has revolutionized the visualization of nanoscale subcellular structures via bypassing the diffraction limit and has become a powerful tool in biological research. Among various super-resolution techniques, single-molecule localization microscopy (SMLM) offers exceptionally high resolution in the range of 10–100 nm. This review presents a concise overview of the fundamental concepts of SMLM and various types of nanoparticle-based fluorophores that can be utilized for SMLM-based super-resolution bioimaging. First, we summarize the unique characteristics, advantages, and drawbacks of various blinking nanomaterials that are used in SMLM imaging applications. Next, we focus on the applications of these blinking nanomaterials in SMLM-based super-resolution imaging of various biological structures and processes. Finally, we discuss the challenges associated with nanoparticle-based SMLM probes and their potential solutions, providing insights into the advancement of this cutting-edge imaging modality.

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闪烁纳米粒子作为超分辨率生物成像探针：综述

超分辨率显微镜通过绕过衍射极限，彻底改变了纳米级亚细胞结构的可视化，并已成为生物学研究的有力工具。在各种超分辨率技术中，单分子定位显微镜（SMLM）在10-100 nm范围内提供了极高的分辨率。本文简要介绍了SMLM的基本概念和各种基于纳米粒子的荧光团，这些荧光团可用于基于SMLM的超分辨率生物成像。首先，我们总结了用于SMLM成像应用的各种闪烁纳米材料的独特特性，优点和缺点。接下来，我们将重点研究这些闪烁纳米材料在基于smlm的各种生物结构和过程的超分辨率成像中的应用。最后，我们讨论了与基于纳米粒子的SMLM探针相关的挑战及其潜在的解决方案，为这种尖端成像方式的进步提供了见解。

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来源期刊

ACS Applied Nano Materials Multiple-

CiteScore

8.30

自引率

3.40%

发文量

1601

期刊介绍： ACS Applied Nano Materials is an interdisciplinary journal publishing original research covering all aspects of engineering, chemistry, physics and biology relevant to applications of nanomaterials. The journal is devoted to reports of new and original experimental and theoretical research of an applied nature that integrate knowledge in the areas of materials, engineering, physics, bioscience, and chemistry into important applications of nanomaterials.