A sustainable solution for eucalyptus cultivation under environmental stresses: novel Pseudomonas species with plant growth-promoting traits

IF 3.9 2区农林科学 Q1 AGRONOMY

Plant and Soil Pub Date : 2025-05-16 DOI:10.1007/s11104-025-07505-4

Tian Liu, Yuhan Jiang, Weishan Zhang, Lu Lin, Xinlei Fan

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引用次数: 0

Abstract

Background and aims

Eucalyptus has unique wood properties and great economic value, but traditional yield-increasing methods rely on chemicals and harm the environment. Plant growth-promoting rhizobacteria (PGPR) are a good solution to this problem, and Pseudomonas is a typical example. The study aims to explore sustainable, eco-friendly alternatives to minimize chemical pollution of the environment by screening and characterizing Pseudomonas with the potential to promote the growth of eucalyptus.

Methods

The Salkowski colorimetric method and various selective media were used to screen for bacteria with growth-promoting functions. Genes associated with growth-promoting functions were identified by genome annotation. A combination of phylogenetic, comparative genomic, morphological, physiological and biochemical methods was used to classify and identify the strains.

Results

Genomic annotation identified genes associated with growth-promoting functions (e.g., ppk for phosphate solubilization, trpA/B/C for IAA synthesis), consistent with the results of plate experiments, further confirming their growth-promoting potential. The two strains were classified as new species within the Pseudomonas genus, and named Pseudomonas eucalyptica sp. nov. and Pseudomonas eucalyptoterra sp. nov.

Conclusion

This study provides new ideas for the development of green, non-polluting biofertilizers and adds to our knowledge of Pseudomonas diversity.

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环境胁迫下桉树种植的可持续解决方案：具有植物生长促进特性的假单胞菌新品种

背景与目的桉树具有独特的木材性能和巨大的经济价值，但传统的增产方法依赖化学药品，对环境有害。促进植物生长的根瘤菌（PGPR）很好地解决了这一问题，假单胞菌是一个典型的例子。该研究旨在通过筛选和表征具有促进桉树生长潜力的假单胞菌，探索可持续的、环保的替代品，以最大限度地减少对环境的化学污染。方法采用Salkowski比色法和多种选择性培养基筛选具有促生长功能的细菌。通过基因组注释鉴定了与促进生长功能相关的基因。采用系统发育、比较基因组学、形态学、生理生化等综合方法对菌株进行分类鉴定。结果基因组注释鉴定出与促生长功能相关的基因（如ppk用于磷酸溶解，trpA/B/C用于IAA合成），与平板实验结果一致，进一步证实了它们的促生长潜力。这两株菌株被归类为假单胞菌属新种，分别命名为Pseudomonas eucalyptus sp. nov和Pseudomonas eucalyptus sp. nov。结论本研究为绿色无污染生物肥料的开发提供了新的思路，增加了我们对假单胞菌多样性的认识。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant and Soil 农林科学-农艺学

CiteScore

8.20

自引率

8.20%

发文量

543

审稿时长

2.5 months

期刊介绍： Plant and Soil publishes original papers and review articles exploring the interface of plant biology and soil sciences, and that enhance our mechanistic understanding of plant-soil interactions. We focus on the interface of plant biology and soil sciences, and seek those manuscripts with a strong mechanistic component which develop and test hypotheses aimed at understanding underlying mechanisms of plant-soil interactions. Manuscripts can include both fundamental and applied aspects of mineral nutrition, plant water relations, symbiotic and pathogenic plant-microbe interactions, root anatomy and morphology, soil biology, ecology, agrochemistry and agrophysics, as long as they are hypothesis-driven and enhance our mechanistic understanding. Articles including a major molecular or modelling component also fall within the scope of the journal. All contributions appear in the English language, with consistent spelling, using either American or British English.