Electrochemical aptasensor for thrombin based on polyfunctionalized dendritic nanodevice as natural enzyme-free labeling element

IF 8 1区化学 Q1 CHEMISTRY, ANALYTICAL

Sensors and Actuators B: Chemical Pub Date : 2025-05-15 DOI:10.1016/j.snb.2025.137977

Anabel Villalonga , Mihai-Cristian Fera , Alicia Piedras , Alfredo Sánchez , Diana Vilela , Irene Ojeda , Yaiza Belacortu , Paloma Martínez-Ruíz , Reynaldo Villalonga

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Abstract

A novel dendritic hybrid nanomaterial with biorecognition and catalytic properties was synthesized by covalent attachment of specific aptamers on the surface of poly(amidoamine) G-4.5 dendrimers and further in situ growth of Pt nanoparticles encapsulated into the polymeric cavities. This nanodevice was employed as natural enzyme-free labeling element to design an amperometric aptasensor with sandwich-type architecture for the specific detection of thrombin, by using screen-printed carbon electrodes functionalized with Au nanoparticles and capture aptamers as transduction interface. The aptasensor was employed to detect the cardiovascular biomarker in the broad range from 1.0 pM to 1.5 nM, with detection limit of 200 fM. This electroanalytical device also showed high specificity, reproducibility and stability, retaining 92 % of its initial biosensing capacity after 50 days of storage at 4ºC. The aptasensor was successfully employed to quantify thrombin in spiked human serum samples.

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基于多功能化树突状纳米器件作为天然无酶标记元件的凝血酶电化学配体传感器

通过在聚氨基胺G-4.5树状大分子表面配体共价吸附，制备了一种具有生物识别和催化性能的新型枝状杂化纳米材料。该纳米器件作为天然无酶标记元件，采用金纳米粒子功能化的丝网印刷碳电极和捕获适体作为转导界面，设计了具有三明治结构的安培适体传感器，用于特异性检测凝血酶。该传感器在1.0 pM ~ 1.5 nM的宽范围内检测心血管生物标志物，检测限为200 fM。该电分析装置还显示出高特异性、重复性和稳定性，在4ºC下保存50天后，其初始生物传感能力仍保持92%。该配体传感器成功地用于定量加标人血清样品中的凝血酶。

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来源期刊

Sensors and Actuators B: Chemical 工程技术-电化学

CiteScore

14.60

自引率

11.90%

发文量

1776

审稿时长

3.2 months

期刊介绍： Sensors & Actuators, B: Chemical is an international journal focused on the research and development of chemical transducers. It covers chemical sensors and biosensors, chemical actuators, and analytical microsystems. The journal is interdisciplinary, aiming to publish original works showcasing substantial advancements beyond the current state of the art in these fields, with practical applicability to solving meaningful analytical problems. Review articles are accepted by invitation from an Editor of the journal.