A Thermus thermophilus Argonaute (TtAgo) cleavage-aided isothermal amplification strategy (TAC-IAS) for precise detection of locus-specific DNA methylation†

IF 4.3 2区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Chemical Communications Pub Date : 2025-06-02 DOI:10.1039/d5cc02012c

Fangfang Sun , Bingjie Han , Yi Zhao , Xing Chen , Hui Tian , Chenghui Liu , Yuanyuan Sun

引用次数: 0

Abstract

A versatile TtAgo cleavage-aided isothermal amplification strategy (TAC-IAS) is devised for precise detection of DNA methylation with high flexibility and universality compared to methylation-restriction endonuclease, reliably identifying methylation with an abundance of 0.1% in a large pool of unmethylated fragments.

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热嗜热菌Argonaute （TtAgo）切割辅助等温扩增策略（TAC-IAS）精确检测位点特异性DNA甲基化

与甲基化限制性内切酶相比，设计了一种多功能TtAgo切割辅助等温扩增策略（TAC-IAS），用于DNA甲基化精确检测，具有高灵活性和通用性，在大量未甲基化片段中可靠地鉴定甲基化，富集度为0.1%。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Chemical Communications 化学-化学综合

CiteScore

8.60

自引率

4.10%

发文量

2705

审稿时长

1.4 months

期刊介绍： ChemComm (Chemical Communications) is renowned as the fastest publisher of articles providing information on new avenues of research, drawn from all the world''s major areas of chemical research.