Hybrid surface layers with antibacterial and anti-inflammatory activity for implants materials

IF 15.8 1区材料科学 Q1 METALLURGY & METALLURGICAL ENGINEERING

Journal of Magnesium and Alloys Pub Date : 2025-05-16 DOI:10.1016/j.jma.2025.04.014

Mariia A. Piatkova, Konstantine V. Nadaraia, Arina I. Ponomarenko, Igor V. Manzhulo, Mariia S. Gerasimenko, Arina I. Pleshkova, Evgeny A. Belov, Igor M. Imshinetskiy, Ksenia A. Fomenko, Ivan S. Osmushko, Aleksey G. Kozlov, Sergey V. Gnedenkov, Dmitry V. Mashtalyar

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Abstract

Currently, there is an essential need for bioresorbable bone implants with antibacterial, anti-inflammatory properties as well as osteoinductivity. Considering this, in presented study for the first time hybrid Mg-ZK coatings with polydopamine (PDA), menaquinone-7 (MK-7), zoledronate (ZA) and vancomycin on the hydroxyapatite (HA) containing layer are formed. Porous coatings were obtained by the plasma electrolytic oxidation (PEO) on an Mg alloy, then MK-7 was impregnated into the pores and PDA film with ZA and vancomycin was polymerized on the samples surface. The presence of HA and organic bioactive compounds was confirmed by EDS, Raman spectroscopy, XRD and XPS. Surface free energy values of hybrid coatings are close to optimal for cell adhesion: 75.28±1.35 mJ/m². Viability tests of the medium, in which Mg-ZK implants were soaked, revealed cytotoxic activity on human osteosarcoma cells with no such an effect on fibroblasts. Antibacterial tests showed an inhibition zone on S. aureus with no viable colonies on the hybrid coatings. The growth inhibition zones for the samples with the hybrid coating were 21±1 mm. Data of electrochemical impedance spectroscopy shows increase of corrosion resistance of samples with hybrid coating by 7 times comparing the Mg alloy without a coating. These properties make the hybrid Mg-ZK coating an attractive modification for bone implants.

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具有抗菌和抗炎活性的植入材料杂化表面层

目前，迫切需要具有抗菌、抗炎和成骨性的生物可吸收骨植入物。因此，本研究首次在含羟基磷灰石（HA）层上形成了聚多巴胺（PDA）、甲基萘醌-7 （MK-7）、唑来膦酸钠（ZA）和万古霉素的Mg-ZK杂化膜。通过等离子体电解氧化（PEO）在镁合金表面制备多孔涂层，然后将MK-7浸渍在多孔涂层中，并在表面聚合ZA和万古霉素。通过能谱分析、拉曼光谱分析、XRD和XPS分析证实了有机活性物质的存在。混合涂层的表面自由能值接近于细胞粘附的最佳值：75.28±1.35 mJ/m2。Mg-ZK植入物浸泡的培养基的活力测试显示，对人骨肉瘤细胞有细胞毒活性，对成纤维细胞没有这种作用。抑菌试验表明，复合膜对金黄色葡萄球菌有抑制作用，但无活菌落。杂化涂层对样品的生长抑制区为21±1 mm。电化学阻抗谱数据表明，与未涂覆的镁合金相比，涂覆复合镀层的耐蚀性提高了7倍。这些特性使得混合Mg-ZK涂层成为一种有吸引力的骨植入物改性材料。

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来源期刊

Journal of Magnesium and Alloys Engineering-Mechanics of Materials

CiteScore

20.20

自引率

14.80%

发文量

审稿时长

59 days

期刊介绍： The Journal of Magnesium and Alloys serves as a global platform for both theoretical and experimental studies in magnesium science and engineering. It welcomes submissions investigating various scientific and engineering factors impacting the metallurgy, processing, microstructure, properties, and applications of magnesium and alloys. The journal covers all aspects of magnesium and alloy research, including raw materials, alloy casting, extrusion and deformation, corrosion and surface treatment, joining and machining, simulation and modeling, microstructure evolution and mechanical properties, new alloy development, magnesium-based composites, bio-materials and energy materials, applications, and recycling.