Differential Genes Analysis and Validation of Disulfideptosis in Rat Myocardial Ischemia-Reperfusion Injury Model.

IF 1.2 4区综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES

Jove-Journal of Visualized Experiments Pub Date : 2025-04-18 DOI:10.3791/68019

Qingxian Tu, Wei Li

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Abstract

Myocardial ischemia-reperfusion injury (MIRI) is an additional injury that occurs during the process of restoring heart tissue blood flow after ischemia-induced injury. MIRI seriously affects the efficacy and short-term and long-term prognosis of reperfusion after myocardial infarction. At present, the mechanism of MIRI is not fully clear. Disulfideptosis is a novel mode of cell death, and the relationship between MIRI and disulfideptosis-related genes (DRGs) expression is still unclear. Firstly, this study explores the differentially expressed genes associated with disulfideptosis in MIRI through bioinformatics analysis. Secondly, by constructing a rat model of MIRI, DRGs were further detected. This study identified 12 related genes, including Myh9, SLC7A11, SLC3A2, Myh7b, ACTB, FLNB, Actn1, Actn4, Flnc, Dbn1 and Pdlim1. Myocardial tissue of rats with MIRI shows obvious pathological damage and apoptosis events. The results of immunohistochemistry indicated that MIRI stimulation increased the expression of GLUT1 protein in myocardial tissue but restricted the expression of F-actin protein. In addition, significant differences in the expression of three proteins were validated using external datasets and MIRI rat models. This study demonstrated that DRGs had significant predictive value in MIRI, providing new prospects for exploring biomarkers and potential therapeutic targets of MIRI.

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大鼠心肌缺血-再灌注损伤模型二硫沉积差异基因分析及验证。

心肌缺血再灌注损伤（心肌缺血再灌注损伤）是缺血损伤后心脏组织血流恢复过程中发生的一种附加损伤。MIRI严重影响心肌梗死后再灌注的疗效和短期、长期预后。目前，MIRI的发病机制尚不完全清楚。二硫沉积是一种新的细胞死亡模式，MIRI与二硫沉积相关基因（DRGs）表达之间的关系尚不清楚。首先，本研究通过生物信息学分析探讨了MIRI中与二硫沉积症相关的差异表达基因。其次，通过构建大鼠MIRI模型，进一步检测DRGs。本研究共鉴定出Myh9、SLC7A11、SLC3A2、Myh7b、ACTB、FLNB、Actn1、Actn4、Flnc、Dbn1、Pdlim1等12个相关基因。MIRI大鼠心肌组织出现明显的病理损伤和凋亡事件。免疫组化结果显示，MIRI刺激心肌组织GLUT1蛋白表达增加，F-actin蛋白表达受限。此外，使用外部数据集和MIRI大鼠模型验证了三种蛋白表达的显着差异。本研究表明DRGs在MIRI中具有显著的预测价值，为探索MIRI的生物标志物和潜在治疗靶点提供了新的前景。

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来源期刊

Jove-Journal of Visualized Experiments MULTIDISCIPLINARY SCIENCES-

CiteScore

2.10

自引率

0.00%

发文量

992

期刊介绍： JoVE, the Journal of Visualized Experiments, is the world''s first peer reviewed scientific video journal. Established in 2006, JoVE is devoted to publishing scientific research in a visual format to help researchers overcome two of the biggest challenges facing the scientific research community today; poor reproducibility and the time and labor intensive nature of learning new experimental techniques.