Response of leaf internal CO2 concentration and intrinsic water-use efficiency in Norway spruce to century-long gradual CO2 elevation.

Abstract

The strategies of Norway spruce [Picea abies (L.) Karst.] to increasing atmospheric CO₂ concentration (C _a) are not entirely clear. Here, we reconstructed centennial trajectories of leaf internal CO₂ concentration (C _i) and intrinsic water-use efficiency (WUE_i) from the amount of ¹³C in tree-ring cellulose. We collected 57 cores across elevations, soil, and atmospheric conditions in central Europe. Generally, WUE_i and C _i increased over the last 100 years and the C _i/C _a ratio remained almost constant. However, two groups were distinguished. The first group showed a quasi-linear response to C _a and the sensitivity of C _i to C _a (s = dC _i/dC _a) ranged from 0 to 1. Trees in the second group showed nonmonotonic responses with extremes during the peak of industrial air pollution in the 1980s and s increase from -1 to +1.6. Our study shows a marked attenuation of the rise in WUE_i during the 20^th century leading to invariant WUE_i in recent decades.