Toward handheld optically guided biopsy combining diffuse reflectance spectroscopy and autofluorescence.

IF 3 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Journal of Biomedical Optics Pub Date : 2025-05-01 Epub Date: 2025-05-06 DOI:10.1117/1.JBO.30.5.057001

Lotte M de Roode, Simon T Sørensen, Stefan D van der Stel, Lisanne L de Boer, Yineng Wang, Huihui Lu, Stefan Andersson-Engels, Theo J M Ruers, Ray Burke

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引用次数: 0

Abstract

Significance: We aim to validate the technologies essential for a handheld, optically guided biopsy device designed to enhance the diagnostic yield and accuracy of percutaneous liver biopsy procedures.

Aim: We aim to combine diffuse reflectance spectroscopy (DRS) and autofluorescence (AF) spectroscopy in a fiber-optic needle probe using mini spectrometers for the classification of tumor and healthy tissues.

Approach: A fiber-optic needle probe combining DRS and AF spectroscopy, incorporating mini spectrometers to facilitate future integration into a biopsy actuator, was designed and built. This custom probe was used to measure healthy liver tissues and colorectal metastases in excised liver segments. A linear discriminant analysis was applied to the DRS and AF data to distinguish tumors from healthy tissues.

Results: The miniaturized combined DRS and AF spectroscopy system could accurately distinguish tumors from healthy liver with a sensitivity of 95% and a specificity of 96% from a sample size of $N = 10$ patients and 52 measurements.

Conclusions: We demonstrate the feasibility of miniaturizing a combined DRS and AF spectroscopy system for the classification of tumor and healthy tissues. This validation supports the feasibility and further development of a handheld, optically guided biopsy device.

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手持式光学引导活检结合漫反射光谱和自身荧光。

意义：我们旨在验证手持式光学引导活检设备的基本技术，该设备旨在提高经皮肝活检程序的诊断率和准确性。目的：将漫反射光谱（DRS）和自身荧光光谱（AF）结合在光纤针探针上，利用微型光谱仪对肿瘤和健康组织进行分类。方法：设计并制造了一种结合DRS和AF光谱的光纤针探针，并结合了微型光谱仪，以方便将来集成到活检驱动器中。这种定制探针用于测量健康肝组织和切除肝段的结肠直肠转移。对DRS和AF数据进行线性判别分析，以区分肿瘤和健康组织。结果：在N = 10例患者和52次测量的样本中，小型化的DRS和AF联合光谱系统能够准确地区分肿瘤和健康肝脏，灵敏度为95%，特异性为96%。结论：我们证明了小型化DRS和AF联合光谱系统用于肿瘤和健康组织分类的可行性。该验证支持了手持式光学引导活检设备的可行性和进一步开发。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Biomedical Optics 医学-光学

CiteScore

6.40

自引率

5.70%

发文量

263

审稿时长

2 months

期刊介绍： The Journal of Biomedical Optics publishes peer-reviewed papers on the use of modern optical technology for improved health care and biomedical research.