Interactions between time on diet, antibiotic treatment, and liver abscess development on the fecal microbiome of beef cattle.

IF 4.9 Q1 MICROBIOLOGY
Germán Plata, Nielson T Baxter, Troy B Hawkins, Lucas Huntimer, Akshitha Nagireddy, Dwi Susanti, James B Reinbold
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引用次数: 0

Abstract

Background: Liver abscesses caused by polymicrobial infections of the liver are a widespread problem in feedlot cattle production. There are currently no effective methods for the early detection of liver abscesses or to predict antibiotic efficacy for their control. Although gene expression and microbiome differences have been reported in the rumen of abscessed and normal animals, liver abscess biomarkers using less invasive tools can facilitate managing of the disease in the field.

Results: Here we report the results of two studies measuring the fecal microbiome composition of steers that did or did not develop liver abscesses, with or without antibiotic treatment, along a 7-month feeding period on a high-concentrate diet. Our results indicate a limited impact of liver abscesses or tylosin on fecal microbiome composition, with time on diet explaining most variance in the fecal microbiome. Interestingly, in both studies, antibiotic treatment led to larger differences in the variability of the fecal microbiomes between abscessed and normal animals compared to controls. These differences were limited to specific sampling times in each of the two studies. Although multiple amplicon sequence variants with differential abundances according to liver abscess state were identified, there was no overlap between the two studies.

Conclusions: Our results suggest that the fecal abundance of individual microorganisms may not be a robust predictor of liver abscess susceptibility across sampling times or diet regimes. Fecal biomarkers of liver abscess susceptibility might be developed with a focus on other aspects of the hindgut microbiome, especially for animals receiving preventive antibiotics.

饮食时间、抗生素治疗和肉牛粪便微生物群中肝脓肿发展之间的相互作用。
背景:由肝脏多微生物感染引起的肝脓肿是饲养场牛生产中普遍存在的问题。目前还没有有效的方法来早期发现肝脓肿或预测抗生素的控制效果。虽然在脓肿动物和正常动物的瘤胃中已经报道了基因表达和微生物组的差异,但使用侵入性较小的工具的肝脓肿生物标志物可以促进现场疾病的管理。结果:在这里,我们报告了两项研究的结果,测量了有或没有发生肝脓肿的阉牛的粪便微生物组成,使用或不使用抗生素治疗,沿着7个月的高精料饲粮喂养期。我们的研究结果表明,肝脓肿或泰洛菌素对粪便微生物组组成的影响有限,饮食时间解释了粪便微生物组的大部分差异。有趣的是,在这两项研究中,与对照组相比,抗生素治疗导致脓肿动物和正常动物粪便微生物组变异性的差异更大。这些差异仅限于两项研究中的特定采样时间。虽然发现了多个扩增子序列变异,其丰度根据肝脓肿状态有所不同,但两项研究之间没有重叠。结论:我们的研究结果表明,粪便中单个微生物的丰度可能不是一个可靠的预测肝脓肿易感性的采样时间或饮食方案。肝脓肿敏感性的粪便生物标志物可能会随着后肠道微生物组其他方面的关注而发展,特别是对于接受预防性抗生素治疗的动物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
0.00%
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审稿时长
13 weeks
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