Exploring the Relationship Between Primary Sarcopenia and Intestinal Barrier Dysfunction in Geriatric Patients: Insights from SARC-F, Serum DAO, Zonulin, LPS, and I-FABP Levels.

IF 3.1 3区医学 Q3 GERIATRICS & GERONTOLOGY

Gerontology Pub Date : 2025-04-22 DOI:10.1159/000545939

Seyda Bilgin, Veysel Suzan, Bahar Bektan Kanat, Gulru Ulugerger Avci, Suna Avci, Hakan Yavuzer, Ibrahim Murat Bolayırlı, Alper Doventas, Deniz Suna Erdincler

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引用次数: 0

Abstract

Introduction: The objective of this study is to assess the correlation between serum levels of DAO (Diamine oxidase), LPS (Lipopolysaccharide), I-FABP (Intestinal Fatty Acid Binding Protein), and Zonulin with primary sarcopenia in elderly patients, and to compare the diagnostic performance of these biomarkers with the SARC-F score.

Methods: The study included 88 patients aged 65 and above. Comorbidities and geriatric syndromes were assessed, and those with secondary sarcopenia were excluded. The EWGSOP2 criteria were applied for diagnosing sarcopenia, while the SARC-F questionnaire was utilized to identify individuals at risk of sarcopenia. Serum levels of DAO, Zonulin, LPS, and I-FABP were measured using ELISA.

Results: No significant differences were observed between the two groups regarding age and gender. SARC-F, serum DAO, and Zonulin concentrations were notably higher in the sarcopenia group than in the non-sarcopenia group (p<0.001). Statistically significant parameters were analyzed using stepwise multiple regression. In stepwise multivariate logistic regression analysis, both DAO and Zonulin scores were found to be associated with sarcopenia [Odds ratio (OR) 1.041, (95%) confidence interval (CI) 1.020-1.063, p<0.001; OR 1.108, (95%) CI 1.018-1.206, p=0.018, subsequently]. ROC curve analysis revealed that the area under the curve (AUC) for DAO, Zonulin, and SARC-F was 0.807 (p<0.001), 0.723 (p<0.001), and 0.943 (p<0.001), subsequently. The Spearman correlation test revealed a significant, low-correlation between serum Zonulin and the SARC-F score (r = 0.3583, p=0.0006), and a moderate-correlation between serum DAO and the SARC-F score (r = 0.4384, p<0.0001).

Conclusion: This study is the first to show a connection between primary sarcopenia and intestinal barrier dysfunction. While the findings support the use of the SARC-F questionnaire in routine practice, serum levels of DAO and Zonulin could serve as an alternative when the SARC-F questionnaire is not applicable.

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探讨老年患者原发性肌肉减少症与肠屏障功能障碍之间的关系：来自SARC-F、血清DAO、Zonulin、LPS和I-FABP水平的见解

本研究的目的是评估老年患者血清中DAO（二胺氧化酶）、LPS（脂多糖）、I-FABP（肠道脂肪酸结合蛋白）和Zonulin水平与原发性肌少症的相关性，并将这些生物标志物的诊断性能与SARC-F评分进行比较。方法：研究对象为年龄在65岁及以上的88例患者。评估合并症和老年综合征，排除继发性肌肉减少症患者。EWGSOP2标准用于诊断肌少症，而SARC-F问卷用于识别肌少症风险个体。采用ELISA法测定血清DAO、Zonulin、LPS、I-FABP水平。结果：两组在年龄和性别上无显著差异。肌少症组的SARC-F、血清DAO和Zonulin浓度明显高于非肌少症组(结论：本研究首次揭示了原发性肌少症与肠屏障功能障碍之间的联系。虽然研究结果支持在常规实践中使用SARC-F问卷，但当SARC-F问卷不适用时，血清DAO和Zonulin水平可以作为替代方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gerontology 医学-老年医学

CiteScore

6.00

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： In view of the ever-increasing fraction of elderly people, understanding the mechanisms of aging and age-related diseases has become a matter of urgent necessity. ''Gerontology'', the oldest journal in the field, responds to this need by drawing topical contributions from multiple disciplines to support the fundamental goals of extending active life and enhancing its quality. The range of papers is classified into four sections. In the Clinical Section, the aetiology, pathogenesis, prevention and treatment of agerelated diseases are discussed from a gerontological rather than a geriatric viewpoint. The Experimental Section contains up-to-date contributions from basic gerontological research. Papers dealing with behavioural development and related topics are placed in the Behavioural Science Section. Basic aspects of regeneration in different experimental biological systems as well as in the context of medical applications are dealt with in a special section that also contains information on technological advances for the elderly. Providing a primary source of high-quality papers covering all aspects of aging in humans and animals, ''Gerontology'' serves as an ideal information tool for all readers interested in the topic of aging from a broad perspective.