Epigenetic Modulation of Cisplatin Sensitivity by the M6A-Linked ceRNA Network in Non-Small Cell Lung Cancer.

IF 5 2区生物学 Q2 CELL BIOLOGY

American journal of physiology. Cell physiology Pub Date : 2025-04-28 DOI:10.1152/ajpcell.00881.2024

Qi Wang, He Yan, Jing Zhang, Jie Zhang, Xiaomin Su, Zhenzhong Su

引用次数: 0

Abstract

Cisplatin resistance significantly impedes effective treatment of non-small cell lung cancer (NSCLC). This study investigates the role of the M6A-related circFUT8/miR-185-5p/HNRNPC competing endogenous RNA (ceRNA) axis in NSCLC cisplatin resistance. Bioinformatics analysis identified HNRNPC, a critical M6A modification-related gene, as a promoter of NSCLC proliferation and metastasis. Our in vitro and in vivo experiments reveal that circFUT8 upregulates HNRNPC by sponging miR-185-5p, thus enhancing NSCLC cell proliferation, migration, and invasion while reducing apoptosis and sensitivity to cisplatin. These findings highlight the circFUT8/miR-185-5p/HNRNPC axis as a potential target to overcome chemoresistance in NSCLC.

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m6a连接的ceRNA网络在非小细胞肺癌中顺铂敏感性的表观遗传学调控。

顺铂耐药严重阻碍非小细胞肺癌（NSCLC）的有效治疗。本研究探讨了m6a相关cirfut8 /miR-185-5p/HNRNPC竞争内源性RNA （ceRNA）轴在NSCLC顺铂耐药中的作用。生物信息学分析发现HNRNPC是一个关键的M6A修饰相关基因，是NSCLC增殖和转移的启动子。我们的体外和体内实验表明，cirfut8通过海绵miR-185-5p上调HNRNPC，从而增强NSCLC细胞的增殖、迁移和侵袭，同时减少细胞凋亡和顺铂敏感性。这些发现强调cirfut8 /miR-185-5p/HNRNPC轴是克服NSCLC化疗耐药的潜在靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

American journal of physiology. Cell physiology 生物-生理学

CiteScore

9.10

自引率

1.80%

发文量

252

审稿时长

1 months

期刊介绍： The American Journal of Physiology-Cell Physiology is dedicated to innovative approaches to the study of cell and molecular physiology. Contributions that use cellular and molecular approaches to shed light on mechanisms of physiological control at higher levels of organization also appear regularly. Manuscripts dealing with the structure and function of cell membranes, contractile systems, cellular organelles, and membrane channels, transporters, and pumps are encouraged. Studies dealing with integrated regulation of cellular function, including mechanisms of signal transduction, development, gene expression, cell-to-cell interactions, and the cell physiology of pathophysiological states, are also eagerly sought. Interdisciplinary studies that apply the approaches of biochemistry, biophysics, molecular biology, morphology, and immunology to the determination of new principles in cell physiology are especially welcome.