Evaluation of a Novel Streptomyces avermitilis Strain from Egyptian Soil for Biological Control of Tetranychus urticae in Greenhouse Cucumber Plantations.

Norhan Saied, Rabaa Yaseen, Amal Abdelmonem Mostafa, Marwa El-Desouky Barghout, Rasha Hussein Ahmed, Basma Hamdy Amin, Mohamed Abdelalim Ali
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Abstract

Background: Infestation in greenhouse cucumber with the two-spotted spider mite (Tetranychus urticae Koch) commonly causes severe damage to crop quality and quantity and increases crop production costs.

Objective: This study was conducted to investigate the efficacy of high-abamectinproducing isolates of S. avermitilis against T. urticae-infested cucumber and to assess their impact on biochemical stress markers in these vegetables.

Methods: In this study, 72 non-antagonistic Streptomyces were isolated from rhizospheric soil samples collected from eight different locations in Egypt and screened for their ability to produce the secondary metabolite, abamectin.

Results: The screening process identified two potent abamectin-producing isolates, EW8 and T2, which produced 42.7 and 29.6 μg/L abamectin, respectively, as confirmed by LC-MS/MS analysis. According to DNA sequence analysis of the 16S rRNA gene, these two isolates belong to the species S. avermitilis. The acaricidal activity of either culture suspensions of S. avermitilis strains WE8 and T2, or their extracts containing abamectin, against the mobile stages and egg hatchability of T. urticae was evaluated in the laboratory and the greenhouse. Data on the mortality among the examined female mites and the reduction in their number of eggs point out a potential acaricidal activity of the examined strains of S. avermitilis and their extracts containing abamectin against T. urticaes. Furthermore, the extracts containing abamectin from these two S. avermitilis strains induced oxidative stress in the infested cucumber plants by T. urticaes, as indicated by increased levels of malondialdehyde (MDA). However, the levels of MDA in T. urticae-infested cucumber plants varied depending on the strain and the specific abamectin crude extract used.

Conclusion: S. avermitilis strains T2 or WE8, or their crude extract could be applied in greenhouse cucumber plantations to combat red mite infestation.

一株埃及土壤阿维链霉菌对温室黄瓜叶螨的生物防治效果评价。
背景:双斑蜘蛛螨(Tetranychus urticae Koch)侵染温室黄瓜,严重危害作物的质量和数量,增加了作物的生产成本。目的:研究高产阿维菌素的阿维杆菌分离株对荨麻疹菌侵染黄瓜的抑菌效果,并评价其对黄瓜生化胁迫指标的影响。方法:本研究从埃及8个不同地点的根际土壤样品中分离出72株非拮抗链霉菌,并对其产生次生代谢产物阿维菌素的能力进行筛选。结果:通过LC-MS/MS分析,筛选到2株产阿维菌素强效菌株EW8和T2,产阿维菌素量分别为42.7和29.6 μg/L。根据16S rRNA基因的DNA序列分析,这两株分离物属于阿维菌属。在室内和温室试验中,研究了阿维菌素提取物和阿维菌素菌株WE8和T2培养悬浮液对荨麻疹流动期和卵孵化率的杀螨活性。对所检测的雌螨死亡率和卵数减少的数据表明,所检测的阿维菌素及其提取物对荨麻疹螨具有潜在的杀螨活性。此外,含有阿维菌素的两种阿维菌素提取物可诱导受感染的黄瓜植株受到荨麻疹杆菌的氧化应激,其表现为丙二醛(MDA)水平升高。然而,在被荨麻疹菌感染的黄瓜植株中,丙二醛的水平因菌株和使用的特定阿维菌素粗提物而异。结论:阿维杆菌T2、WE8或其粗提物可用于温室黄瓜田防治红螨。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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