Integration of acoustic, optical, and electrical methods in picoliter droplet microfluidics for rare particles enrichment.

Huasheng Zhuo, Chunhua He, Canfeng Yang, Xian Jiang, Fan Li, Xiangliang Yang, Hai Yang, Tuying Yong, Zhiyong Liu, Yan Ma, Lei Nie, Guanglan Liao, Tielin Shi
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Abstract

Rare particle enrichment plays a pivotal role in advancing numerous scientific research areas and industrial processes. Traditional enrichment methods encounter obstacles such as low efficiency, high cost, and complexity. Acoustic focusing, optical fiber detection, and electrical manipulation have shown potential in microfluidics for particle manipulation and analysis. This study pioneers the integration of the acoustic, optical, and electrical units to overcome the traditional limitations. The cooperative dynamics of acoustic and flow focusing are explored. The optical fibers with an enhanced detection algorithm greatly boost optical detection sensitivity. Furthermore, the droplet charging to enhance the tip charging phenomenon is complemented and validated. The detection and sorting accuracy of enriching large-size H22 cells reached 99.8% and 99.3%, respectively, with the target cell concentration increased by nearly 86-fold. Our work significantly enhances detection sensitivity and particle manipulation accuracy, ultimately offering a robust and reliable solution for generating droplets to enrich rare particles.

集成声学、光学和电学方法在皮升液滴微流体中富集稀有粒子。
稀有粒子富集在推进许多科学研究领域和工业过程中起着举足轻重的作用。传统的富集方法存在效率低、成本高、操作复杂等问题。声学聚焦、光纤检测和电操作在微流体中显示了粒子操作和分析的潜力。这项研究开创了声学、光学和电气单元的集成,以克服传统的局限性。探讨了声聚焦与流聚焦的协同动力学。改进了检测算法的光纤大大提高了光检测的灵敏度。此外,还补充并验证了液滴充能增强尖端充能现象。富集大尺寸H22细胞的检测和分选准确率分别达到99.8%和99.3%,靶细胞浓度提高了近86倍。我们的工作显著提高了检测灵敏度和粒子操作精度,最终为生成液滴富集稀有粒子提供了一种强大可靠的解决方案。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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