The crucial role of cerebrospinal fluid cytology in the diagnosis and prognosis of medulloblastoma at M1 stage.

IF 0.8 4区 医学 Q4 CLINICAL NEUROLOGY
Nithye Parvathy, Neha Bhardwaj, Radhika Srinivasan, Nalini Gupta, Parikshaa Gupta, Manish Rohilla, Reetu Kundu, Pranab Dey, Kirti Gupta, Nandita Kakkar, Amita Trehan, Paramjeet Singh, Renu Madan, Pravin Salunke
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引用次数: 0

Abstract

Introduction: Medulloblastoma is the most common pediatric malignant embryonal tumor of the cerebellum. In the absence of radiologically proven metastasis, lumbar puncture with cytological examination of the cerebrospinal fluid (CSF) is mandatory for identification of the M1 stage. This study aims to evaluate CSF cytomorphology and prognosis of the M1 stage.

Materials and methods: A retrospective 6-year audit (2017 - 2023) was performed for all cases of medulloblastoma on histopathology (n = 303). CSF cytology was evaluated in 177 cases on 2 routinely prepared smears after cytocentrifugation. A detailed evaluation of cytomorphological features and corresponding histopathology was performed and correlated with outcome in M1 stage cases (n = 18).

Results: Out of 177 cases of histopathology-proven medulloblastoma, CSF cytology was reported as positive for infiltration in 18 cases (14 classical and 4 desmoplastic variants) and were assigned M1 stage. The median age of the patients was 7.5 years. CSF smear showed high cellularity with malignant cell clusters of more than 200 cells in 8 cases, whereas 4 cases had low cellularity with scattered cells and admixed with inflammatory cells. Tumor cells showed a high nucleocytoplasmic ratio, coarse chromatin, and prominent nuclear molding. Nucleoli were inconspicuous in 9 cases but were prominent and eosinophilic in 9 cases. The median overall survival (OS) and progression-free survival (PFS) in M1 stage medulloblastoma was poor, 2 months and 1 month, respectively. There was a difference in age and tumor histology among the M0, M1, and M2/3 stage medulloblastomas.

Conclusion: CSF infiltration by medulloblastoma cells characterized by high nucleocytoplasmic ratio, nuclear molding, and coarse chromatin, represents the M1 stage and portends a poor prognosis.

脑脊液细胞学检查在M1期髓母细胞瘤的诊断和预后中的重要作用。
髓母细胞瘤是儿童小脑最常见的恶性胚胎性肿瘤。在没有放射学证实转移的情况下,腰椎穿刺和脑脊液(CSF)细胞学检查是确定M1期的必要条件。本研究旨在评价M1期脑脊液细胞形态学和预后。材料和方法:对所有髓母细胞瘤病例(n = 303)进行回顾性6年(2017 - 2023)的组织病理学检查。对177例患者行2张常规涂片,经细胞离心后行脑脊液细胞学检查。对M1期病例(n = 18)的细胞形态学特征和相应的组织病理学进行了详细的评估,并将其与预后相关联。结果:在177例经组织病理学证实的髓母细胞瘤中,18例脑脊液细胞学报告浸润阳性(14例典型和4例纤维增生性变异体),并被划分为M1期。患者的中位年龄为7.5岁。脑脊液涂片显示高细胞性,8例可见200多个恶性细胞团,4例可见低细胞性,细胞分散,并伴有炎性细胞。肿瘤细胞核质比高,染色质粗,核成型明显。9例核仁不明显,9例核仁明显且嗜酸性。M1期髓母细胞瘤的中位总生存期(OS)和无进展生存期(PFS)较差,分别为2个月和1个月。M0期、M1期和M2/3期髓母细胞瘤在年龄和肿瘤组织学上存在差异。结论:髓母细胞瘤细胞浸润脑脊液表现为核质比高、核成型、染色质粗,为M1期,预后较差。
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来源期刊
Clinical Neuropathology
Clinical Neuropathology 医学-病理学
CiteScore
1.60
自引率
0.00%
发文量
70
审稿时长
>12 weeks
期刊介绍: Clinical Neuropathology appears bi-monthly and publishes reviews and editorials, original papers, short communications and reports on recent advances in the entire field of clinical neuropathology. Papers on experimental neuropathologic subjects are accepted if they bear a close relationship to human diseases. Correspondence (letters to the editors) and current information including book announcements will also be published.
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