Loperamide increases mouse gut transit time in a dose-dependent manner with treatment duration-dependent effects on distinct gut microbial taxa.

Gut microbiome (Cambridge, England) Pub Date : 2025-05-02 eCollection Date: 2025-01-01 DOI:10.1017/gmb.2025.5
Anna Pii Hjørne, Martin Steen Mortensen, Tine Rask Licht, Martin Frederik Laursen
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Abstract

Intestinal transit time has been recognized as an important factor in shaping the gut microbiota, although causality remains to be firmly demonstrated. The aim of this study was to evaluate the effect of different loperamide doses on the mouse intestinal transit time and to investigate the effects of increasing transit time on the gut microbial community. Loperamide significantly increased the transit time in a dose-dependent manner. Additionally, we observed a significant difference between the control group and the loperamide-treated groups in the abundance of the bacterial families Bacteroidaceae, Erysipelotrichaceae, Porphyromonadaceae, and Akkermansiaceae after 7 days of loperamide treatment, with the bacterial families responding to the increased transit time at different rates. Fermentation of faeces obtained from the same mice, with or without loperamide, demonstrated that the observed effects on gut microbiota in vivo were not a result of direct interactions between loperamide and the gut microbiota but rather a consequence of loperamide-induced increased intestinal transit time. In the cecum of the mice, we found higher levels of propionate in the high-dose group compared to the control and low-dose groups. Collectively, our findings establish that an altered transit time is causal to changes in the composition and activity of the microbiome.

洛哌丁胺以剂量依赖的方式增加小鼠肠道转运时间,并对不同的肠道微生物类群产生治疗持续时间依赖的影响。
肠道运输时间已被认为是塑造肠道微生物群的重要因素,尽管因果关系仍有待证实。本研究的目的是评估不同剂量洛哌丁胺对小鼠肠道转运时间的影响,并探讨增加转运时间对肠道微生物群落的影响。洛哌丁胺以剂量依赖的方式显著增加了传递时间。此外,我们观察到,在洛哌丁胺处理7天后,对照组与洛哌丁胺处理组在Bacteroidaceae, Erysipelotrichaceae, Porphyromonadaceae和Akkermansiaceae细菌科的丰度存在显著差异,并且细菌科对运输时间增加的反应速度不同。从同一只小鼠身上获得的粪便发酵,无论是否使用洛哌丁胺,都表明观察到的对体内肠道微生物群的影响不是洛哌丁胺和肠道微生物群直接相互作用的结果,而是洛哌丁胺诱导肠道运输时间增加的结果。在小鼠的盲肠中,我们发现高剂量组的丙酸水平高于对照组和低剂量组。总的来说,我们的研究结果表明,传递时间的改变是导致微生物组组成和活性变化的原因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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