[MSB-15] Histopathological Comparison of the Effectiveness of St. Thomas II and del Nido Cardioplegia Solutions with Respect to Myocardial Ischemia and Reperfusion in Rats.
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Abstract
Objective: This study aimed to compare the effectiveness of traditional St. Thomas II and the next-generation del Nido cardioplegia solutions in minimizing ischemia/reperfusion injury during open heart surgery in a myocardial ischemia/reperfusion model created in rats.
Methods: In this experimental study, 24 Wistar albino rats were divided into three groups: the sham group (normal saline), the St. Thomas II group, and the del Nido group. Cardioplegia was induced, and heart tissues were collected for histopathological analysis after 90 min. The number of macrophages was counted to assess inflammation.
Results: The mean macrophage count in ischemic tissues was similar across the sham, St. Thomas II, and del Nido groups. In tissues with reperfusion injury, the control group had fewer macrophages compared to the St. Thomas II and del Nido groups, but the difference between the two solutions was not statistically significant.
Conclusion: Both St. Thomas II and del Nido solutions were more effective than the control in preventing reperfusion injury. However, no significant difference was observed between the two cardioplegia solutions, possibly due to the short ischemia/reperfusion duration and the resilient nature of rat myocardium.
[MSB-15] St. Thomas II和del Nido停搏液对大鼠心肌缺血再灌注效果的组织病理学比较。
目的：本研究旨在比较传统St. Thomas II和新一代del Nido停搏液在心脏直视手术心肌缺血再灌注模型中减少缺血再灌注损伤的效果。方法：将24只Wistar白化大鼠分为假手术组（生理盐水）、St. Thomas II组和del Nido组。诱导心脏停跳，90 min后采集心脏组织进行组织病理学分析，计数巨噬细胞数量评估炎症反应。结果：假手术组、St. Thomas II组和del Nido组缺血组织中巨噬细胞的平均计数相似。在再灌注损伤组织中，对照组巨噬细胞数量少于St. Thomas II和del Nido组，但两种溶液间差异无统计学意义。结论：St. Thomas II和del Nido溶液对再灌注损伤的预防效果均优于对照组。然而，两种停搏液之间没有明显差异，可能是由于缺血/再灌注时间短和大鼠心肌的弹性。
期刊介绍：
The Turkish Journal of Thoracic and Cardiovascular Surgery is an international open access journal which publishes original articles on topics in generality of Cardiac, Thoracic, Arterial, Venous, Lymphatic Disorders and their managements. These encompass all relevant clinical, surgical and experimental studies, editorials, current and collective reviews, technical know-how papers, case reports, interesting images, How to Do It papers, correspondences, and commentaries.
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