Community-Wide Genotyping of Batrachochytrium dendrobatidis in Ecuadorian Forests.

IF 2.2 3区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Ecohealth Pub Date : 2025-05-05 DOI:10.1007/s10393-025-01716-y

Wesley J Neely, M D M Moretta-Urdiales, Utpal Smart, Ryan L Lynch, Juan Manuel Guayasamin, Shawn F McCracken, David Rodriguez

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引用次数: 0

Abstract

The amphibian chytrid fungus Batrachochytrium dendrobatidis (Bd) is a cosmopolitan pathogen with numerous distinct lineages. The global panzootic lineage (Bd-GPL) is the most widespread and virulent lineage and is responsible for many recorded amphibian declines. Mapping the extent of Bd-GPL and other more established lineages is important for predicting disease dynamics in amphibian communities. Ecuador is the most biodiverse country per unit area for amphibian taxa and, thus, a priority for studies on genotypic diversity of Bd. In this study, we tested skin swab samples collected from 464 individual amphibians across coastal, Andean montane, and Amazonian forests, for the presence of two Bd lineages known to be present in South America: the globally-distributed Bd-GPL and the Brazilian-endemic Bd-Asia2/Brazil lineage. By using a discriminatory qPCR SNP assay, we found widespread prevalence of Bd-GPL in Ecuador in diverse host taxa. Genotyping efficiency was 36% in this study, meaning that one in every three swabs that tested positive for Bd in infection assays were successfully genotyped. Through this study, we provide further support for the presence of a single Bd lineage in this neotropical biodiversity hotspot.

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厄瓜多尔森林树突壶菌群落基因分型研究。

两栖壶菌水蛭壶菌（Batrachochytrium dendroatidis, Bd）是一种具有许多不同谱系的世界性病原体。全球流行病谱系（Bd-GPL）是最广泛和毒性最强的谱系，是造成许多两栖动物数量下降的原因。绘制Bd-GPL和其他更成熟的谱系的范围对于预测两栖动物群落的疾病动态非常重要。厄瓜多尔是单位面积两栖动物群生物多样性最丰富的国家，因此是研究Bd基因型多样性的优先选择。在本研究中，我们测试了从沿海、安第斯山脉和亚马逊森林中收集的464只两栖动物的皮肤样本，以确定存在于南美洲的两种已知Bd谱系：全球分布的Bd- gpl和巴西特有的Bd-亚洲a2/巴西谱系。通过使用歧视性qPCR SNP测定，我们发现Bd-GPL在厄瓜多尔的不同宿主分类群中广泛流行。在这项研究中，基因分型效率为36%，这意味着每三个在感染试验中检测出Bd阳性的拭子中就有一个成功地进行了基因分型。通过这项研究，我们进一步支持了单一Bd谱系在这个新热带生物多样性热点地区的存在。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ecohealth 环境科学-环境科学

CiteScore

4.50

自引率

4.00%

发文量

审稿时长

>24 weeks

期刊介绍： EcoHealth aims to advance research, practice, and knowledge integration at the interface of ecology and health by publishing high quality research and review articles that address and profile new ideas, developments, and programs. The journal’s scope encompasses research that integrates concepts and theory from many fields of scholarship (including ecological, social and health sciences, and the humanities) and draws upon multiple types of knowledge, including those of relevance to practice and policy. Papers address integrated ecology and health challenges arising in public health, human and veterinary medicine, conservation and ecosystem management, rural and urban development and planning, and other fields that address the social-ecological context of health. The journal is a central platform for fulfilling the mission of the EcoHealth Alliance to strive for sustainable health of people, domestic animals, wildlife, and ecosystems by promoting discovery, understanding, and transdisciplinarity. The journal invites substantial contributions in the following areas: One Health and Conservation Medicine o Integrated research on health of humans, wildlife, livestock and ecosystems o Research and policy in ecology, public health, and agricultural sustainability o Emerging infectious diseases affecting people, wildlife, domestic animals, and plants o Research and practice linking human and animal health and/or social-ecological systems o Anthropogenic environmental change and drivers of disease emergence in humans, wildlife, livestock and ecosystems o Health of humans and animals in relation to terrestrial, freshwater, and marine ecosystems Ecosystem Approaches to Health o Systems thinking and social-ecological systems in relation to health o Transdiiplinary approaches to health, ecosystems and society.