{"title":"Optimizing amplification threshold of low template DNA","authors":"Ayelet Ido MSc, Lina Kirshenbaum PhD, Ortal Waiskopf MSc, Lev Voskoboinik PhD","doi":"10.1111/1556-4029.70044","DOIUrl":null,"url":null,"abstract":"<p>Forensic DNA laboratories often face challenges in processing low template DNA samples efficiently. This study aimed to optimize the criteria for amplifying low-quantity samples by considering DNA concentration, degradation factor, and biological sample type. We analyzed 155 real forensic case samples with DNA concentrations between 5 and 14.3 pg/μL using the PowerPlex® ESI 16 Fast System. Samples were categorized based on DNA concentration, degradation index, and predicted success rate. We found that 27% of profiles were informative, with higher success rates for samples with DNA concentrations above 10 pg/μL and degradation index below 3. By implementing these empirically established thresholds, laboratories can potentially reduce workload by 32% while retaining 83% of informative profiles in low template samples. This approach can help forensic laboratories balance the need for comprehensive evidence processing with resource constraints.</p>","PeriodicalId":15743,"journal":{"name":"Journal of forensic sciences","volume":"70 4","pages":"1521-1526"},"PeriodicalIF":1.8000,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of forensic sciences","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/1556-4029.70044","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, LEGAL","Score":null,"Total":0}
引用次数: 0
Abstract
Forensic DNA laboratories often face challenges in processing low template DNA samples efficiently. This study aimed to optimize the criteria for amplifying low-quantity samples by considering DNA concentration, degradation factor, and biological sample type. We analyzed 155 real forensic case samples with DNA concentrations between 5 and 14.3 pg/μL using the PowerPlex® ESI 16 Fast System. Samples were categorized based on DNA concentration, degradation index, and predicted success rate. We found that 27% of profiles were informative, with higher success rates for samples with DNA concentrations above 10 pg/μL and degradation index below 3. By implementing these empirically established thresholds, laboratories can potentially reduce workload by 32% while retaining 83% of informative profiles in low template samples. This approach can help forensic laboratories balance the need for comprehensive evidence processing with resource constraints.
期刊介绍：
The Journal of Forensic Sciences (JFS) is the official publication of the American Academy of Forensic Sciences (AAFS). It is devoted to the publication of original investigations, observations, scholarly inquiries and reviews in various branches of the forensic sciences. These include anthropology, criminalistics, digital and multimedia sciences, engineering and applied sciences, pathology/biology, psychiatry and behavioral science, jurisprudence, odontology, questioned documents, and toxicology. Similar submissions dealing with forensic aspects of other sciences and the social sciences are also accepted, as are submissions dealing with scientifically sound emerging science disciplines. The content and/or views expressed in the JFS are not necessarily those of the AAFS, the JFS Editorial Board, the organizations with which authors are affiliated, or the publisher of JFS. All manuscript submissions are double-blind peer-reviewed.
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