Cytosolic glyceraldehyde-3-phosphate dehydrogenase regulates plant stem cell maintenance under oxidative stress.

IF 5.3 2区生物学 Q1 PLANT SCIENCES

Plant Cell Reports Pub Date : 2025-05-13 DOI:10.1007/s00299-025-03507-9

Jiaqi Qiu, Minghuang Chen, Zheqi Cai, Xiaofen Chen, Zelong Pang, Hao Chen, Tao Huang

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引用次数: 0

Abstract

Key message: GAPDH regulates plant stem cell maintenance. WUSCHEL (WUS) and WUSCHEL-RELATED HOMEOBOX (WOX) family proteins are vital for maintaining the homeostasis of stem cells, which is necessary for the continuous growth and the development of plants. Plants frequently encounter environmental stress that can lead to an increase in reactive oxygen species, such as hydrogen peroxide (H₂O₂). However, the exact ways in which plant stem cells sense and respond to H₂O₂ signals remain unclear. This research indicates that cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH) helps regulate stem cell maintenance in Arabidopsis in response to H₂O₂. Hydrogen peroxide causes the relocation of two cytosolic GAPDH proteins, GAPC1 and GAPC2, from the cytoplasm to the nucleus. These isoforms interact with WUS/WOX proteins and modulate the expression of the WUS/WOX gene by binding to its promoter. When the expression of GAPC1 and GAPC2 is decreased, stem cell homeostasis and overall plant growth become more sensitive to H₂O₂. Thus, cytosolic GAPDH may serve as a sensor for H₂O₂, influencing the maintenance of plant stem cells under oxidative stress.

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胞质甘油醛-3-磷酸脱氢酶调控植物干细胞在氧化胁迫下的维持。

关键信息：GAPDH调节植物干细胞的维持。WUSCHEL （WUS）和WUSCHEL相关HOMEOBOX （WOX）家族蛋白对维持干细胞的内稳态至关重要，是植物持续生长发育所必需的。植物经常会遇到环境压力，这会导致活性氧的增加，比如过氧化氢（H2O2）。然而，植物干细胞感知和响应H2O2信号的确切方式尚不清楚。本研究表明，胞质甘油醛-3-磷酸脱氢酶（GAPDH）有助于调节拟南芥干细胞对H2O2的维持。过氧化氢导致两种细胞质GAPDH蛋白，GAPC1和GAPC2，从细胞质转移到细胞核。这些同工异构体与WUS/WOX蛋白相互作用，并通过结合其启动子来调节WUS/WOX基因的表达。当GAPC1和GAPC2表达降低时，干细胞稳态和植物整体生长对H2O2更加敏感。因此，胞质GAPDH可能作为H2O2的传感器，影响植物干细胞在氧化胁迫下的维持。

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来源期刊

Plant Cell Reports 生物-植物科学

CiteScore

10.80

自引率

1.60%

发文量

135

审稿时长

3.2 months

期刊介绍： Plant Cell Reports publishes original, peer-reviewed articles on new advances in all aspects of plant cell science, plant genetics and molecular biology. Papers selected for publication contribute significant new advances to clearly identified technological problems and/or biological questions. The articles will prove relevant beyond the narrow topic of interest to a readership with broad scientific background. The coverage includes such topics as: - genomics and genetics - metabolism - cell biology - abiotic and biotic stress - phytopathology - gene transfer and expression - molecular pharming - systems biology - nanobiotechnology - genome editing - phenomics and synthetic biology The journal also publishes opinion papers, review and focus articles on the latest developments and new advances in research and technology in plant molecular biology and biotechnology.