Exploring alternative therapeutic options for multidrug-resistant N. Gonorrhoeae: the potential of 7,8-dihydroxyflavone.

IF 3.6 3区医学 Q2 INFECTIOUS DISEASES

Sexually Transmitted Infections Pub Date : 2025-05-13 DOI:10.1136/sextrans-2024-056362

Yuqiu Qi, Qinghui Xie, Jingxia Lin, Ran Zhang, Yingxian Tang, Zhuo Wang, Xiaomian Lin

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引用次数: 0

Abstract

Objectives: Neisseria gonorrhoeae (NG), a bacterium characterised by multidrug resistance, is traditionally treated with ceftriaxone, either as a monotherapy or in conjunction with azithromycin. However, the emergence of strains resistant to these antibiotics presents a considerable challenge to current therapeutic approaches, which necessitates the exploration of alternative treatment strategies.

Methods: Agar dilution and broth microdilution to determine the minimum inhibitory concentrations (MICs) of antibiotics and 7,8-dihydroxyflavone. The development of 7,8-dihydroxyflavone drug resistance was detected under the treatment of sublethal concentration for 30 passages. Genotypic analysis was conducted to examine penA, 23S rRNA alleles and multilocus sequence typing.

Results: 55 clinical NG isolates emphasised the high-level rates of the current antimicrobial resistance, with a notable prevalence of the penA-60.001 FC428 clone and high-level azithromycin-resistant clones characterised by the 23S rRNA-1 (A2059G) and 23S rRNA-2 (C2611T) alleles. Among the strains that are representative of clinical circulation, 7,8-dihydroxyflavone demonstrated inhibitory activity, with MIC₅₀ and MIC₉₀ values of 10 mg/L and 20 mg/L, respectively, which are unlikely to induce drug resistance in NG.

Conclusions: 7,8-dihydroxyflavone may represent a promising antimicrobial agent in the context of escalating antibiotic resistance.

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探索耐多药淋病奈瑟菌的替代治疗方案：7,8-二羟黄酮的潜力。

目的：淋病奈瑟菌（NG）是一种以多药耐药为特征的细菌，传统上用头孢曲松治疗，要么作为单一疗法，要么与阿奇霉素联合治疗。然而，对这些抗生素耐药菌株的出现对当前的治疗方法提出了相当大的挑战，这需要探索替代治疗策略。方法：琼脂稀释法和微量肉汤稀释法测定抗生素和7,8-二羟黄酮的最低抑菌浓度（mic）。在亚致死浓度处理30代下，检测了7,8-二羟黄酮耐药的发展情况。进行基因型分析，检测penA、23S rRNA等位基因及多位点序列分型。结果：55株临床NG分离株当前抗菌素耐药率较高，其中penA-60.001 FC428克隆和23S rRNA-1 （A2059G）和23S rRNA-2 （C2611T）等位基因的高水平阿奇霉素耐药克隆的患病率显著。在具有临床循环代表性的菌株中，7,8-二羟黄酮表现出抑制活性，MIC50和MIC90值分别为10 mg/L和20 mg/L，不太可能引起NG耐药。结论：7,8-二羟黄酮可能是一种有前途的抗菌剂，在抗生素耐药性不断升级的背景下。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Sexually Transmitted Infections 医学-传染病学

CiteScore

5.70

自引率

8.30%

发文量

审稿时长

4-8 weeks

期刊介绍： Sexually Transmitted Infections is the world’s longest running international journal on sexual health. It aims to keep practitioners, trainees and researchers up to date in the prevention, diagnosis and treatment of all STIs and HIV. The journal publishes original research, descriptive epidemiology, evidence-based reviews and comment on the clinical, public health, sociological and laboratory aspects of sexual health from around the world. We also publish educational articles, letters and other material of interest to readers, along with podcasts and other online material. STI provides a high quality editorial service from submission to publication.