Chamber design and intensity-modulated ultraviolet-C LEDs for advanced pulsed photonic disinfection.

Biomedizinische Technik. Biomedical engineering Pub Date : 2025-05-06 DOI:10.1515/bmt-2025-0070

Tan Tian Swee, Jahanzeb Sheikh, Syafiqah Saidin, Jose-Javier Serrano Olmedo, Sidra Abid Agha, Maheza Irna Binti Salim

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Abstract

Objectives: Contaminated apparatus and surgical tools pose serious health risks. For such purpose, disinfection chambers are employed. However, these systems rely on mercury-based UV lamps which comes with various drawbacks. These limitations have driven interest in Ultraviolet-C Light Emitting Diode (UV-C LED) technology as a safer and more efficient alternative. However, existing studies have not thoroughly explored the impact of varying intensities of pulse width modulation (PWM) on disinfection efficacy.

Methods: To addess this, the present study designed and tested a LED-based disinfection chamber by employing 4-W 275 nm Surface Mount Device (SMD) LEDs against frequently isolated bacteria. By following prior approach, irradiation time was alternated at 30-s intervals and antibacterial efficacy was assessed through various parameters. Additionally, scanning electron microscopy (SEM) was performed to examine the morphological changes.

Results: Results indicated that the reduction was significantly influenced (p<0.05) with varying PWM levels (60-100 %), achieving 2.05-log₁₀ and 1.54-log₁₀ inactivation against Escherichia coli and Staphylococcus aureus, respectively, upon exposure to 51.24 mJ/cm² under maximum exposure settings. Moreover, complete cellular damage leading to bleb protrusion and cell-leakage confirmed the disruption of bacterial DNA.

Conclusions: In conclusion, UV-LEDs show great potential for disinfection, with efficiency influenced by PWM and dosage.

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用于先进脉冲光子消毒的室设计和强度调制紫外- c led。

目的：受污染的器械和手术工具构成严重的健康风险。为此，使用消毒室。然而，这些系统依赖于汞基紫外线灯，这有各种各样的缺点。这些限制促使人们将UV-C发光二极管（UV-C LED）技术作为一种更安全、更高效的替代技术。然而，现有研究尚未深入探讨不同强度脉宽调制（PWM）对消毒效果的影响。方法：为了解决这一问题，本研究设计并测试了一个基于led的消毒室，该消毒室采用4-W 275 nm表面贴装器件（SMD） led对经常被分离的细菌进行消毒。按照之前的方法，每隔30秒交替照射时间，并通过各种参数评估抗菌效果。此外，扫描电子显微镜（SEM）检查形态学变化。结果：结果表明，在最大暴露设置下，暴露于51.24 mJ/cm2时，对大肠杆菌和金黄色葡萄球菌的失活分别有显著影响（p10和1.54 log10）。此外，导致水泡突出和细胞渗漏的完全细胞损伤证实了细菌DNA的破坏。结论：紫外- led具有很大的消毒潜力，其消毒效果受PWM和剂量的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedizinische Technik. Biomedical engineering

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