Temporal microbial colonization on different forages is driven by the rumen environmental conditions.

IF 4.9 Q1 MICROBIOLOGY
X Xie, J K Wang, J X Liu, L L Guan, A L A Neves
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Abstract

The rumen is one of the four compartments of the ruminant stomach and houses a diverse array of anaerobic microbes that play a crucial role in feed digestion and volatile fatty acid (VFA) production. The aim of this study was to explore how two different in vivo rumen environmental conditions, AHR (created from sheep-fed alfalfa hay) and CSR (created from sheep-fed corn stover), affect fiber digestion and rumen bacterial colonization in relation to two types of forage, alfalfa hay (AH) and corn stover (CS). Both AH and CS forages were subjected to in-sacco incubation in AHR and CSR conditions for a period of 48 h. The results revealed that CSR exhibited a less variant pH, lower total VFA concentration, and higher acetate-to-propionate ratio than AHR. CSR significantly enhanced the degradation of neutral detergent fiber and acid detergent fiber in both incubated forages (AH and CS). Although CSR did not improve the degradation of dry matter (DM) or crude protein (CP) on AH, it improved the degradation of DM and CP on CS. Both CS and AH incubated under CSR were found to have a greater abundance of fibrolytic bacteria (e.g., Fibrobacter and Butyrivibrio 2) compared to the same forage incubated under AHR, especially during the initial stages of incubation. However, CS and AH incubated under AHR were colonized by bacteria specialized in breaking down soluble carbohydrates (e.g., Prevotella and Succinivibrio). Compared with AHR, CSR enhanced the degradation rates of both incubated forages (CS and AH). These findings underscore the role of the rumen microenvironment in affecting the composition of adherent microbial communities and enhancing the breakdown of forages. Therefore, optimizing the rumen microenvironment to promote the attachment of fibrolytic bacteria during the early fermentation stages while minimizing hydrogen accumulation to stabilize the pH could lead to improved forage fermentation and animal performance.

瘤胃环境条件决定了微生物在不同饲料上的时间定殖。
瘤胃是反刍动物胃的四个区室之一,是各种厌氧微生物的栖息地,这些微生物在饲料消化和挥发性脂肪酸(VFA)的产生中起着至关重要的作用。本研究的目的是探讨两种不同的体内瘤胃环境条件,AHR(由羊饲苜蓿干草产生)和CSR(由羊饲玉米秸秆产生),如何影响苜蓿干草(AH)和玉米秸秆(CS)两种饲料对纤维消化和瘤胃细菌定植的影响。在AHR和CSR条件下,AH和CS两种牧草均在甘蔗内孵育48 h。结果表明,与AHR相比,CSR的pH值变化较小,总VFA浓度较低,乙酸/丙酸比较高。CSR显著提高了培养牧草(AH和CS)中中性洗涤纤维和酸性洗涤纤维的降解。虽然CSR没有提高干物质(DM)和粗蛋白质(CP)在AH上的降解,但提高了DM和CP在CS上的降解。与AHR下孵育的相同饲料相比,在CSR下孵育的CS和AH具有更丰富的纤维分解细菌(例如,纤维杆菌和丁酸弧菌2),特别是在孵育的初始阶段。然而,在AHR条件下培养的CS和AH被专门分解可溶性碳水化合物的细菌定植(如普雷沃氏菌和琥珀酸弧菌)。与AHR相比,CSR提高了培养牧草(CS和AH)的降解率。这些发现强调了瘤胃微环境在影响附着微生物群落组成和促进牧草分解方面的作用。因此,优化瘤胃微环境,在发酵前期促进纤维分解菌的附着,同时减少氢积累,稳定pH,可以提高饲料发酵和动物生产性能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
7.20
自引率
0.00%
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审稿时长
13 weeks
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