Cryptochrome-mediated blue light regulates cell lignification via PbbHLH195 activation of the PbNSC in pear fruits.

IF 10.6 Q1 HORTICULTURE

Molecular Horticulture Pub Date : 2025-05-07 DOI:10.1186/s43897-025-00149-z

Qi Wang, Xinyi Wu, Mei Ren, Fanghang Zhang, Yang Zhang, Yueyang Wang, Wen Li, Zhihua Xie, Kaijie Qi, Shaoling Zhang, Katsuhiro Shiratake, Yingying Niu, Shutian Tao

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引用次数: 0

Abstract

The presence of stone cells in pear fruit, caused by lignified secondary cell walls (SCWs), leads to a grainy texture in the fruit flesh, thereby compromising its overall quality. Lignification is influenced by various environmental signals, including light, however the underlying mechanism are poorly understood. This study reveals that SCW thickening and lignin accumulation in stone cells were regulated by a blue light signal, mediated through the activation of PbNSC by PbbHLH195. The results revealed that the stone cell formation was prompted by supplementary with blue light, with lignin accumulation linked to the upregulation of the NAC STONE CELL PROMOTING FACTOR (PbNSC). PbbHLH195 was identified as a novel molecular hub connecting lignification to blue light signal through its physical interaction with PbCRY1a. The biochemical and functional analysis indicates that PbbHLH195 contributes to stone cell lignification by activating the promoter of PbNSC. Our findings offer novel insights into the mechanisms of lignin biosynthesis in response to blue light, identifying valuable genetic targets for enhancing the fruit quality of pear.

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隐色素介导的蓝光通过PbbHLH195激活梨果实PbNSC调控细胞木质化。

由于次生细胞壁木质化（SCWs），梨果实中存在石细胞，导致果肉呈颗粒状，从而影响其整体质量。木质素化受包括光在内的各种环境信号的影响，但其潜在机制尚不清楚。该研究表明，PbbHLH195通过激活PbNSC介导蓝光信号调控石细胞中SCW增厚和木质素积累。结果表明，蓝光的补充促进了石细胞的形成，木质素的积累与NAC stone cell PROMOTING FACTOR （PbNSC）的上调有关。PbbHLH195通过与PbCRY1a的物理相互作用，被鉴定为连接木质化与蓝光信号的新型分子枢纽。生化和功能分析表明PbbHLH195通过激活PbNSC启动子参与石细胞木质化。我们的研究结果为木质素在蓝光下的生物合成机制提供了新的见解，确定了提高梨果实品质的有价值的遗传靶点。

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来源期刊

Molecular Horticulture horticultural research-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Aims Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field. Scope Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants): ▪ Developmental and evolutionary biology ▪ Physiology, biochemistry and cell biology ▪ Plant-microbe and plant-environment interactions ▪ Genetics and epigenetics ▪ Molecular breeding and biotechnology ▪ Secondary metabolism and synthetic biology ▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome. The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest. In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.