Histochemical and Immunohistochemical Identification of Mast Cells in the Rat Lacrimal Glands.

IF 1.7 4区农林科学 Q2 VETERINARY SCIENCES

Veterinary ophthalmology Pub Date : 2025-04-30 DOI:10.1111/vop.70005

Ozkan Yavas

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引用次数: 0

Abstract

Objective: This study aims to investigate the presence, microanatomical localization, and immunohistochemical subtypes of mast cells in the lacrimal glands (LGs) of rats.

Animals: A total of ten female Sprague-Dawley rats' LGs were evaluated in this study.

Procedure(s): The experimental animals were euthanized. LG samples were collected and fixed in 10% neutral formaldehyde for 48 h. Sections were taken from the paraffin-embedded samples and stained for microscopic examination. The histochemical properties of lacrimal gland epithelium and the composition of tears were examined by Periodic acid-Schiff and Alcian blue staining and evaluated microscopically. Toluidine blue staining and tryptase and chymase immunohistochemically demonstrated localization and phenotypes of mast cells.

Results: The existence and varying numbers of mast cells were successfully demonstrated in all three LGs by Toluidine blue staining, and the highest number of mast cells was observed in the extraorbital lacrimal gland, while their numbers were almost the same in the intraorbital lacrimal gland and Harderian gland. Immunohistochemically, chymase-positive mast cells were more numerous than tryptase-positive cells.

Conclusions: This study is the first documentation of the presence of mast cells with different phenotypes in rat LGs.

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大鼠泪腺肥大细胞的组织化学和免疫组织化学鉴定。

目的：研究大鼠泪腺肥大细胞的存在、显微解剖定位和免疫组织化学亚型。实验动物：选取10只雌性Sprague-Dawley大鼠进行LGs测定。收集LG样品，在10%中性甲醛中固定48 h。从石蜡包埋的样品上取切片，染色进行显微镜检查。采用周期酸-希夫染色和阿利新蓝染色观察各组泪腺上皮组织化学性质及泪液组成。甲苯胺蓝染色、胰蛋白酶和乳糜酶免疫组织化学显示了肥大细胞的定位和表型。结果：甲苯胺蓝染色成功证实了肥大细胞的存在和数量的变化，其中眶外泪腺肥大细胞数量最多，眶内泪腺和哈德氏腺肥大细胞数量基本相同。免疫组化结果显示，乳糜酶阳性肥大细胞数量多于胰酶阳性肥大细胞。结论：本研究首次证实了大鼠LGs中存在不同表型的肥大细胞。

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来源期刊

Veterinary ophthalmology 农林科学-兽医学

CiteScore

2.70

自引率

37.50%

发文量

审稿时长

12-24 weeks

期刊介绍： Veterinary Ophthalmology is a peer-reviewed, international journal that welcomes submission of manuscripts directed towards academic researchers of veterinary ophthalmology, specialists and general practitioners with a strong ophthalmology interest. Articles include those relating to all aspects of: Clinical and investigational veterinary and comparative ophthalmology; Prospective and retrospective studies or reviews of naturally occurring ocular disease in veterinary species; Experimental models of both animal and human ocular disease in veterinary species; Anatomic studies of the animal eye; Physiological studies of the animal eye; Pharmacological studies of the animal eye.