Downregulation of huntingtin-associated protein 1 predicts poor prognosis in gastric cancer.

IF 2.6 Q3 ONCOLOGY

World journal of clinical oncology Pub Date : 2025-04-24 DOI:10.5306/wjco.v16.i4.102958

Xiang-Yang Wang, Fu-Hai Yang, Zhong-Yuan Yuan, Zhong-Jing Wang, Hong-Feng Zhang, Zi-Hui Xu

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引用次数: 0

Abstract

Background: Highly expressed in the gastrointestinal mucosa, huntingtin-associated protein 1 (HAP1) is closely associated with tumor development and prognosis.

Aim: To investigate the clinical utility of HAP1 expression in gastric cancer (GC).

Methods: We randomly selected 124 GC patients had not undergone preoperative radiotherapy or chemotherapy, they were diagnosed at the Central Hospital of Wuhan between May 2013 and October 2018. Immunohistochemistry was used to detect HAP1 expression in paraffin-embedded GC tissues, as well as metastatic lymph nodes. Their clinical data were collected and all participants were follow up for 5 years. Western blotting and quantitative polymerase chain reaction were used to detect HAP1 levels in 20 matched pairs of fresh GC tissues.

Results: HAP1 protein and mRNA levels were lower in fresh GC tissues than in normal mucosal tissues (P < 0.001, respectively). Immunohistochemistry also revealed lower HAP1 expression in GC tissues and metastatic lymph nodes than in normal mucosal tissues (P < 0.05). HAP1 expression in GC was closely associated with differentiation, lymph node metastasis, lymph node ratio, remote metastasis, clinical stage, tumor location, and survival time (P < 0.05). Furthermore, HAP1 expression independently predicted GC (P < 0.05) and was more accurate in advanced GC than in early GC (P < 0.05).

Conclusion: HAP1 is an important prognostic biomarker for GC, with low HAP1 expression positively correlating with poor overall survival, especially in advanced clinical stages.

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亨廷顿蛋白相关蛋白1的下调预示胃癌预后不良。

背景：huntingtin-associated protein 1 （HAP1）在胃肠道粘膜中高表达，与肿瘤的发展和预后密切相关。目的：探讨HAP1基因在胃癌组织中的表达及其临床意义。方法：随机选择2013年5月至2018年10月在武汉市中心医院确诊的124例术前未接受放疗或化疗的胃癌患者。免疫组织化学检测HAP1在石蜡包埋的GC组织和转移淋巴结中的表达。收集临床资料，随访5年。采用Western blotting和定量聚合酶链反应检测20对匹配的新鲜GC组织HAP1水平。结果：新鲜胃粘膜组织HAP1蛋白和mRNA水平低于正常胃粘膜组织（P < 0.001）。免疫组化显示，胃癌组织和转移性淋巴结中HAP1的表达低于正常粘膜组织（P < 0.05）。胃癌组织中HAP1表达与分化、淋巴结转移、淋巴结比例、远处转移、临床分期、肿瘤位置、生存时间密切相关（P < 0.05）。HAP1表达独立预测胃癌（P < 0.05），晚期胃癌比早期胃癌更准确（P < 0.05）。结论：HAP1是胃癌重要的预后生物标志物，HAP1低表达与总生存率低呈正相关，尤其是在临床晚期。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World journal of clinical oncology ONCOLOGY-

自引率

0.00%

发文量

585

期刊介绍： The WJCO is a high-quality, peer reviewed, open-access journal. The primary task of WJCO is to rapidly publish high-quality original articles, reviews, editorials, and case reports in the field of oncology. In order to promote productive academic communication, the peer review process for the WJCO is transparent; to this end, all published manuscripts are accompanied by the anonymized reviewers’ comments as well as the authors’ responses. The primary aims of the WJCO are to improve diagnostic, therapeutic and preventive modalities and the skills of clinicians and to guide clinical practice in oncology. Scope: Art of Oncology, Biology of Neoplasia, Breast Cancer, Cancer Prevention and Control, Cancer-Related Complications, Diagnosis in Oncology, Gastrointestinal Cancer, Genetic Testing For Cancer, Gynecologic Cancer, Head and Neck Cancer, Hematologic Malignancy, Lung Cancer, Melanoma, Molecular Oncology, Neurooncology, Palliative and Supportive Care, Pediatric Oncology, Surgical Oncology, Translational Oncology, and Urologic Oncology.