Advances in Detection Methods for A-to-I RNA Editing.

IF 6.4 2区 生物学 Q1 CELL BIOLOGY
Yuxi Yang, Masayuki Sakurai
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引用次数: 0

Abstract

Adenosine-to-inosine (A-to-I) RNA editing is a key post-transcriptional modification that influences gene expression and various cellular processes. Advances in sequencing technologies have greatly contributed to the identification of A-to-I editing sites, providing insights into their distribution across coding and non-coding regions. These developments have facilitated the discovery of functionally relevant editing events and have advanced the understanding of their biological roles. This review presents the evolution of methodologies for RNA editing detection and examines recent advances, including chemically-assisted, enzyme-assisted, and quantitative approaches. By evaluating these techniques, we aim to help researchers select the most effective tools for investigating RNA editing and its broader implications in health and disease.

A-to-I RNA编辑检测方法研究进展
腺苷-肌苷(a -to-i) RNA编辑是影响基因表达和各种细胞过程的关键转录后修饰。测序技术的进步极大地促进了A-to-I编辑位点的鉴定,提供了它们在编码区和非编码区分布的见解。这些发展促进了功能相关编辑事件的发现,并促进了对其生物学作用的理解。这篇综述介绍了RNA编辑检测方法的演变,并检查了最近的进展,包括化学辅助、酶辅助和定量方法。通过评估这些技术,我们的目标是帮助研究人员选择最有效的工具来研究RNA编辑及其对健康和疾病的更广泛影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
14.80
自引率
4.10%
发文量
67
审稿时长
6-12 weeks
期刊介绍: WIREs RNA aims to provide comprehensive, up-to-date, and coherent coverage of this interesting and growing field, providing a framework for both RNA experts and interdisciplinary researchers to not only gain perspective in areas of RNA biology, but to generate new insights and applications as well. Major topics to be covered are: RNA Structure and Dynamics; RNA Evolution and Genomics; RNA-Based Catalysis; RNA Interactions with Proteins and Other Molecules; Translation; RNA Processing; RNA Export/Localization; RNA Turnover and Surveillance; Regulatory RNAs/RNAi/Riboswitches; RNA in Disease and Development; and RNA Methods.
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