High Flavonoid Content in Apium graveolens Nanocrystals Improves Colitis in Dextran Sodium Sulfate-induced Colitis Mice.

Q2 Pharmacology, Toxicology and Pharmaceutics

Pharmaceutical nanotechnology Pub Date : 2025-05-12 DOI:10.2174/0122117385358037250415034215

Verda Farida, Chandra Saputra, Ardian Dewangga, Widi Kurniawan, Muhammad Novrizal Abdi Sahid, Cornelia M Keck

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引用次数: 0

Abstract

Aim: To develop medicinal plant nanoparticles as colitis alternative/supplementary therapy.

Background: Limited reports exist on the effectiveness of medicinal plant nanocrystals in treating or preventing colitis.

Objectives: We investigated the effect of canonizing Apium graveolens (AG) on improving dextran sodium sulfate (DSS)- induced (4%) colitis.

Methods: Nanonization was performed via the bead milling process. The nanocrystal product was characterized (i.e., particle size, zeta potential (ZP), polydispersity index (PDI) values) and freezedried. Total flavonoids and phenolic compounds in nanocrystal products were compared with ethanolic extract of AG (AGEE). Anti-colitis activity of AG-nanocrystal water suspensions (AGNS) was compared to AG bulk powder suspensions (AGBS). Colitis severity was determined via physiological, macroscopic, and microscopic colon assessment. In addition, the fecal Enterobacteriaceae population and urine glucose levels were determined.

Results: The AG nanoparticle products are 200-400 nm, with PDI values 0.5-0.6, and ZP values -12 to -20 mV. The total flavonoid and phenolic compounds of AGNS were 115.12±4.32 ppm and 37.11±0.34 ppm, respectively. This value is higher compared to the content in AGEE. AGNS (350 mg/kg) improves physiological (i.e., fecal blood), macroscopic (i.e., length, diameter), and microscopic (i.e., structure and immune cell infiltration) colon conditions in a comparable level to the positive control of 5-aminosalicylic acid (100 mg/kg). AGNS have a compelling ability to restore colon microscopic and Enterobacteriaceae population compared to AGBS (700 mg/kg). AGNS (350 mg/kg) also recovered colon permeability as marked by the lower urine glucose concentration (9.90±0.15 mg/dL) compared to colitis mice (12.43±0.09 mg/dL).

Conclusion: The nanonization of AG contributes to improved anti-colitis activities compared to AGBS. Nanonization of medicinal plants will reduce organic solvent extraction, which supports the sustainable development goals.

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高黄酮含量的荆芥纳米晶体改善右旋糖酐硫酸钠诱导结肠炎小鼠的结肠炎。

目的：开发药用植物纳米颗粒作为结肠炎的替代/补充疗法。背景：关于药用植物纳米晶体治疗或预防结肠炎的有效性的报道有限。目的：观察加药荆芥（AG）对右旋糖酐硫酸钠（DSS）所致（4%）结肠炎的改善作用。方法：采用磨粒法进行纳米化。对纳米晶产物进行了表征（即粒径、ζ电位（ZP）、多分散性指数（PDI）值）并进行了冷冻。比较了AG乙醇提取物纳米晶产品中总黄酮和酚类化合物的含量。比较了AG纳米晶水悬浮液（AGNS）与AG散装粉末悬浮液（AGBS）的抗结肠炎活性。结肠炎的严重程度通过生理、宏观和微观结肠评估来确定。此外，还测定了粪便肠杆菌科菌群和尿糖水平。结果：制备的AG纳米颗粒粒径为200 ~ 400 nm， PDI值为0.5 ~ 0.6，ZP值为-12 ~ -20 mV。AGNS总黄酮和酚类化合物含量分别为115.12±4.32 ppm和37.11±0.34 ppm。该值高于AGEE中的含量。AGNS （350 mg/kg）将生理（即粪血）、宏观（即长度、直径）和微观（即结构和免疫细胞浸润）结肠状况改善到与5-氨基水杨酸阳性对照（100 mg/kg）相当的水平。与AGBS （700 mg/kg）相比，AGNS具有令人信服的恢复结肠显微和肠杆菌科种群的能力。与结肠炎小鼠（12.43±0.09 mg/dL）相比，AGNS （350 mg/kg）也能恢复结肠通透性（9.90±0.15 mg/dL）。结论：与AGBS相比，AG纳米化有助于提高抗结肠炎活性。药用植物的纳米化将减少有机溶剂提取，有利于实现可持续发展目标。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pharmaceutical nanotechnology Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science

CiteScore

4.20

自引率

0.00%

发文量

期刊介绍： Pharmaceutical Nanotechnology publishes original manuscripts, full-length/mini reviews, thematic issues, rapid technical notes and commentaries that provide insights into the synthesis, characterisation and pharmaceutical (or diagnostic) application of materials at the nanoscale. The nanoscale is defined as a size range of below 1 µm. Scientific findings related to micro and macro systems with functionality residing within features defined at the nanoscale are also within the scope of the journal. Manuscripts detailing the synthesis, exhaustive characterisation, biological evaluation, clinical testing and/ or toxicological assessment of nanomaterials are of particular interest to the journal’s readership. Articles should be self contained, centred around a well founded hypothesis and should aim to showcase the pharmaceutical/ diagnostic implications of the nanotechnology approach. Manuscripts should aim, wherever possible, to demonstrate the in vivo impact of any nanotechnological intervention. As reducing a material to the nanoscale is capable of fundamentally altering the material’s properties, the journal’s readership is particularly interested in new characterisation techniques and the advanced properties that originate from this size reduction. Both bottom up and top down approaches to the realisation of nanomaterials lie within the scope of the journal.