Identification of Indigenous Thai Phlegmariurus Genotypic Population by Integrating Morphological and Molecular Studies.

IF 4 2区生物学 Q1 PLANT SCIENCES

Plants-Basel Pub Date : 2025-05-07 DOI:10.3390/plants14091400

Nusanisa Chedao, Avinash Chandra Pandey, Potjamarn Suraninpong

{"title":"Identification of Indigenous Thai Phlegmariurus Genotypic Population by Integrating Morphological and Molecular Studies.","authors":"Nusanisa Chedao, Avinash Chandra Pandey, Potjamarn Suraninpong","doi":"10.3390/plants14091400","DOIUrl":null,"url":null,"abstract":"Phlegmariurus, a diverse genus within the Lycopodiaceae family, has wide diversity in tropical regions, including Thailand. Accurate species delimitation in the tropical clubmoss genus Phlegmariurus is challenged by high morphological plasticity and genetic complexity. This study applied an integrative multilocus approach combining morphometric analysis of 27 complete specimens, 35 Phlegmariurus and one Lycopodiella accessions for AFLP genotyping (926 loci; PIC 0.32), SSR profiling (44 loci; PIC 0.57; expected heterozygosity 0.35), and chloroplast barcoding using rbcL (1308 bp; bootstrap 89-99%) and the psbA-trnH intergenic spacer (308 bp; bootstrap ≥ 94%). A total of 13 were identified as belonging to seven known species, including P. nummulariifolius (NST01, NST15, NST36), P. goebelii (JP04), P. phlegmaria (NST13), P. verticillatus (PHI16), P. squarrosus (NST21, NST22, MY31), P. tetrastichus (NST30), and P. carinatus (MY32, MY33, NST34). Morphological clustering and molecular markers consistently distinguished Phlegmariurus accessions from the Lycopodiella outgroup. Additionally, 19 previously unclassified Phlegmariurus accessions were successfully identified as belonging to the species P. nummulariifolius (NST23), P. goebelii (NST03, JP05, STN12, PNA14, SKA25, CPN26, KRB27, PNA28), P. phlegmaria (NWT07, STN08, NST09, NST10, PHI29), P. squarrosus (NST17), and P. carinatus (PNA06, STN18, CPN19, JP24). Moreover, this study identified three novel lineages (NST02, STN11, NST20) with strong support across datasets. The combination of broad genomic coverage (AFLP), fine-scale allelic resolution (SSR), deep-branch backbone (rbcL), and terminal-branch discrimination (psbA-trnH) yields a robust framework for species identification. These results define clear operational units for conservation prioritization and establish a foundation for marker-assisted development of ornamental Phlegmariurus cultivars.","PeriodicalId":56267,"journal":{"name":"Plants-Basel","volume":"14 9","pages":""},"PeriodicalIF":4.0000,"publicationDate":"2025-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12073110/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plants-Basel","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3390/plants14091400","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}

引用次数: 0

Abstract

Phlegmariurus, a diverse genus within the Lycopodiaceae family, has wide diversity in tropical regions, including Thailand. Accurate species delimitation in the tropical clubmoss genus Phlegmariurus is challenged by high morphological plasticity and genetic complexity. This study applied an integrative multilocus approach combining morphometric analysis of 27 complete specimens, 35 Phlegmariurus and one Lycopodiella accessions for AFLP genotyping (926 loci; PIC 0.32), SSR profiling (44 loci; PIC 0.57; expected heterozygosity 0.35), and chloroplast barcoding using rbcL (1308 bp; bootstrap 89-99%) and the psbA-trnH intergenic spacer (308 bp; bootstrap ≥ 94%). A total of 13 were identified as belonging to seven known species, including P. nummulariifolius (NST01, NST15, NST36), P. goebelii (JP04), P. phlegmaria (NST13), P. verticillatus (PHI16), P. squarrosus (NST21, NST22, MY31), P. tetrastichus (NST30), and P. carinatus (MY32, MY33, NST34). Morphological clustering and molecular markers consistently distinguished Phlegmariurus accessions from the Lycopodiella outgroup. Additionally, 19 previously unclassified Phlegmariurus accessions were successfully identified as belonging to the species P. nummulariifolius (NST23), P. goebelii (NST03, JP05, STN12, PNA14, SKA25, CPN26, KRB27, PNA28), P. phlegmaria (NWT07, STN08, NST09, NST10, PHI29), P. squarrosus (NST17), and P. carinatus (PNA06, STN18, CPN19, JP24). Moreover, this study identified three novel lineages (NST02, STN11, NST20) with strong support across datasets. The combination of broad genomic coverage (AFLP), fine-scale allelic resolution (SSR), deep-branch backbone (rbcL), and terminal-branch discrimination (psbA-trnH) yields a robust framework for species identification. These results define clear operational units for conservation prioritization and establish a foundation for marker-assisted development of ornamental Phlegmariurus cultivars.

查看原文本刊更多论文

结合形态学和分子研究鉴定泰国土生黏痰菌基因型群体。

粘液属是石松科的一个多样化属，在包括泰国在内的热带地区具有广泛的多样性。热带棒藓属的准确种界受到高度形态可塑性和遗传复杂性的挑战。本研究采用多位点综合分析方法，对27份完整标本、35份痰菌标本和1份石松标本进行AFLP基因分型(926个位点；PIC 0.32)， SSR分析(44个位点；图0.57;预期杂合度0.35)，利用rbcL进行叶绿体条形码(1308 bp；bootstrap 89-99%)和psbA-trnH基因间间隔(308 bp；Bootstrap≥94%)。共鉴定出7个已知种13只，分别为nummulariifolius （NST01、NST15、NST36）、P. goebelii （JP04）、P. phlegmaria （NST13）、P. verticillatus （PHI16）、P. squarrosus （NST21、NST22、MY31）、P. tetrastichus （NST30）和P. carinatus （MY32、MY33、NST34）。形态学聚类和分子标记一致地区分了痰柳属和石松属。此外，19份此前未分类的黏痰菌材料被成功鉴定为P. nummulariifolius （NST23）、P. goebelii （NST03、JP05、STN12、PNA14、SKA25、CPN26、KRB27、PNA28）、P. phlegmaria （NWT07、STN08、NST09、NST10、PHI29）、P. squarrosus （NST17）和P. carinatus （PNA06、STN18、CPN19、JP24）。此外，本研究确定了三个新的谱系（NST02, STN11, NST20），在数据集上得到了强有力的支持。广泛的基因组覆盖（AFLP）、精细的等位基因分辨率（SSR）、深分支主干（rbcL）和末端分支识别（psbA-trnH）的结合为物种鉴定提供了一个强大的框架。这些结果明确了保护优先级的操作单元，并为标记辅助开发观赏痰草品种奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Plants-Basel Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics

CiteScore

6.50

自引率

11.10%

发文量

2923

审稿时长

15.4 days

期刊介绍： Plants (ISSN 2223-7747), is an international and multidisciplinary scientific open access journal that covers all key areas of plant science. It publishes review articles, regular research articles, communications, and short notes in the fields of structural, functional and experimental botany. In addition to fundamental disciplines such as morphology, systematics, physiology and ecology of plants, the journal welcomes all types of articles in the field of applied plant science.