Supplementation of the Probiotic LLH135 Reduces Oxidative Stress in a Model of Hemiparkinsonism.

IF 2.7 4区 医学 Q2 CLINICAL NEUROLOGY
Behavioural Neurology Pub Date : 2025-04-10 eCollection Date: 2025-01-01 DOI:10.1155/bn/8401392
Mario E Flores-Soto, Angelica Y Nápoles-Medina, Aldo R Tejeda-Martínez, Josué R Solís-Pacheco, Verónica Chaparro-Huerta, Juan E Gutiérrez-Sevilla, Blanca R Aguilar-Uscanga
{"title":"Supplementation of the Probiotic LLH135 Reduces Oxidative Stress in a Model of Hemiparkinsonism.","authors":"Mario E Flores-Soto, Angelica Y Nápoles-Medina, Aldo R Tejeda-Martínez, Josué R Solís-Pacheco, Verónica Chaparro-Huerta, Juan E Gutiérrez-Sevilla, Blanca R Aguilar-Uscanga","doi":"10.1155/bn/8401392","DOIUrl":null,"url":null,"abstract":"<p><p>Oxidative stress and neuroinflammation are considered as the two main etiological reasons behind idiopathic Parkinson's disease (PD). Nevertheless, the actual treatments are focused on improving motor symptoms by restoring dopamine (DA) presence, leaving said causes unattended. Probiotics could be a promising strategy for the improvement of these physiological features behind the disease and therefore constitute a complementary treatment for those having PD. This study evaluated the effect of the oral administration of a probiotic bacteria mixture from 3 strains of <i>Limosilactobacillus fermentum</i> LH01<i>, Limosilactobacillus reuteri</i> LH03, and <i>Lactiplantibacillus plantarum</i> LH05 (LLH135), of human milk origin, for 4 weeks, on mice under the hemiparkinsonism model of intrastriatal administration of 6-hidroxidopamine (6-OHDA). We measured total antioxidant capacity (TAC), super oxide dismutase (SOD) activity, and 8-deoxyguanosine (8-OHdG) regarding oxidative stress. Concerning neuroinflammation, immunoreactivity for GFAP, IBA-1, and CD68 was measured by immunohistochemistry and the latter markers corroborated in colocalization with immunofluorescence to assess activated microglia. The probiotic mixture diminished the oxidative stress features of SOD activity as well as 8-OHdG generated by the model of hemiparkinsonism. These effects were accompanied as well by the dampening of the glial immunoreactivity and colocalization of IBA-1 and CD68 that were present under the model. Our findings suggest that the administration of the probiotic LLH135 exerts neuroprotective effects by promoting an antioxidant response which could be explained by the modulation of the response from glial cells to dopaminergic neuronal damage induced with 6-OHDA.</p>","PeriodicalId":50733,"journal":{"name":"Behavioural Neurology","volume":"2025 ","pages":"8401392"},"PeriodicalIF":2.7000,"publicationDate":"2025-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12006709/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Behavioural Neurology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/bn/8401392","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/1 0:00:00","PubModel":"eCollection","JCR":"Q2","JCRName":"CLINICAL NEUROLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Oxidative stress and neuroinflammation are considered as the two main etiological reasons behind idiopathic Parkinson's disease (PD). Nevertheless, the actual treatments are focused on improving motor symptoms by restoring dopamine (DA) presence, leaving said causes unattended. Probiotics could be a promising strategy for the improvement of these physiological features behind the disease and therefore constitute a complementary treatment for those having PD. This study evaluated the effect of the oral administration of a probiotic bacteria mixture from 3 strains of Limosilactobacillus fermentum LH01, Limosilactobacillus reuteri LH03, and Lactiplantibacillus plantarum LH05 (LLH135), of human milk origin, for 4 weeks, on mice under the hemiparkinsonism model of intrastriatal administration of 6-hidroxidopamine (6-OHDA). We measured total antioxidant capacity (TAC), super oxide dismutase (SOD) activity, and 8-deoxyguanosine (8-OHdG) regarding oxidative stress. Concerning neuroinflammation, immunoreactivity for GFAP, IBA-1, and CD68 was measured by immunohistochemistry and the latter markers corroborated in colocalization with immunofluorescence to assess activated microglia. The probiotic mixture diminished the oxidative stress features of SOD activity as well as 8-OHdG generated by the model of hemiparkinsonism. These effects were accompanied as well by the dampening of the glial immunoreactivity and colocalization of IBA-1 and CD68 that were present under the model. Our findings suggest that the administration of the probiotic LLH135 exerts neuroprotective effects by promoting an antioxidant response which could be explained by the modulation of the response from glial cells to dopaminergic neuronal damage induced with 6-OHDA.

补充益生菌LLH135可降低偏帕金森模型的氧化应激。
氧化应激和神经炎症被认为是特发性帕金森病(PD)的两个主要病因。然而,实际治疗的重点是通过恢复多巴胺(DA)的存在来改善运动症状,而对上述原因置之不理。益生菌可能是改善疾病背后这些生理特征的一种有希望的策略,因此构成了PD患者的补充治疗。本研究以人乳源性发酵乳酸杆菌LH01、罗伊氏乳酸杆菌LH03、植物乳酸杆菌LH05 (LLH135) 3株益生菌混合菌,对半帕金森模型下6-羟多巴胺(6-OHDA)灌胃小鼠进行了为期4周的研究。我们测量了氧化应激的总抗氧化能力(TAC)、超氧化物歧化酶(SOD)活性和8-脱氧鸟苷(8-OHdG)。对于神经炎症,免疫组织化学测定GFAP、IBA-1和CD68的免疫反应性,后者标记物与免疫荧光共定位证实,以评估活化的小胶质细胞。益生菌混合物降低了偏帕金森模型产生的SOD活性和8-OHdG的氧化应激特征。这些影响还伴随着模型下存在的神经胶质免疫反应性和IBA-1和CD68共定位的抑制。我们的研究结果表明,益生菌LLH135通过促进抗氧化反应发挥神经保护作用,这可以通过调节神经胶质细胞对6-OHDA诱导的多巴胺能神经元损伤的反应来解释。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Behavioural Neurology
Behavioural Neurology 医学-临床神经学
CiteScore
5.40
自引率
3.60%
发文量
52
审稿时长
>12 weeks
期刊介绍: Behavioural Neurology is a peer-reviewed, Open Access journal which publishes original research articles, review articles and clinical studies based on various diseases and syndromes in behavioural neurology. The aim of the journal is to provide a platform for researchers and clinicians working in various fields of neurology including cognitive neuroscience, neuropsychology and neuropsychiatry. Topics of interest include: ADHD Aphasia Autism Alzheimer’s Disease Behavioural Disorders Dementia Epilepsy Multiple Sclerosis Parkinson’s Disease Psychosis Stroke Traumatic brain injury.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信