Detection and characterization of the Dezidougou virus (genus Negevirus) in mosquitoes (Ochlerotatus caspius) collected in the Republic of Sakha (Yakutia).

Q3 Medicine
M A Stepanyuk, S S Legostaev, K V Karelina, N F Timofeeva, K F Emtsova, O V Ohlopkova, O S Taranov, V A Ternovoi, A V Protopopov, V B Loktev, V A Svyatchenko, A P Agafonov
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引用次数: 0

Abstract

Introduction: Monitoring and research on arthropod-borne microorganisms is important. Recently, with the development of next-generation sequencing methods, many previously unknown viruses have been identified in insects.

Aim of the study: Isolation of viruses from mosquitoes sampled in the Republic of Sakha (Yakutia), followed by the study of a new for Russia negevirus isolated from mosquitoes of the species Ochlerotatus caspius, including determination of its complete nucleotide sequence, phylogenetic and virological characteristics.

Materials and methods: Dezidougou virus isolation was performed on C6/36 (Aedes albopictus) cell culture. Electron microscopy was performed using a JEM 1400 electron microscope. Nucleotide sequence screening was performed by NGS on a high-throughput sequencer MiSeq, Illumina (USA). Full genome nucleotide sequence was determined by Sanger sequencing. Phylogenetic analysis was performed using GenBank database, using Vector NTI Advance 11 and MEGA 11 programs.

Results: The virus isolated from mosquitoes replicated efficiently in C6/36 cells, causing their death. However, it did not replicate in the mammalian cell cultures used. The isolated virus did not cause pathologic manifestations in suckling mice when infected intracerebrally. Electron microscopic examination of the purified virus-containing suspension showed the presence of spherical viral particles with a diameter of 45‒55 nm. The results of full genome sequencing identified it as belonging to Dezidougou virus, first isolated in Côte d'Ivoire. The nucleotide sequence of the genome of Yakutsk 2023 strain of Dezidougou virus was deposited in GenBank (PP975071.1).

Conclusion: Dezidougou virus of genus Negevirus was isolated and characterized for the first time in the Russian Federation. Further studies on the prevalence of negeviruses, their virological features, potential importance for public health and their impact on vector competence of vectors are important and promising.

萨哈共和国(雅库特)采蚊中Dezidougou病毒(阴性病毒属)的检测和特征分析
节肢动物微生物的监测与研究具有重要意义。近年来,随着新一代测序方法的发展,在昆虫中发现了许多以前未知的病毒。该研究的目的:从萨哈共和国(雅库特)取样的蚊子中分离病毒,然后研究从caspius Ochlerotatus蚊子中分离的一种新的俄罗斯阴性病毒,包括确定其完整的核苷酸序列、系统发育和病毒学特征。材料与方法:在C6/36(白纹伊蚊)细胞培养上分离德子独沟病毒。采用JEM 1400型电子显微镜进行电镜观察。核苷酸序列筛选采用高通量测序仪MiSeq, Illumina (USA)的NGS。采用Sanger测序法测定全基因组核苷酸序列。系统发育分析使用GenBank数据库,使用Vector NTI Advance 11和MEGA 11程序。结果:从蚊虫体内分离的病毒在C6/36细胞内复制效率高,可导致蚊虫死亡。然而,它不能在哺乳动物细胞培养物中复制。分离的病毒在哺乳小鼠脑内感染时未引起病理表现。纯化后的含病毒悬浮液电镜检查显示存在直径为45-55 nm的球形病毒颗粒。全基因组测序结果确定该病毒属于首次在Côte科特迪瓦分离到的德齐杜古病毒。deziidougou病毒雅库茨克2023株基因组核苷酸序列保存在GenBank (PP975071.1)中。结论:在俄罗斯首次分离到阴性病毒属的德子独沟病毒。进一步研究阴性病毒的流行、病毒学特征、对公共卫生的潜在重要性及其对病媒能力的影响是重要和有希望的。
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来源期刊
Voprosy virusologii
Voprosy virusologii Medicine-Infectious Diseases
CiteScore
2.00
自引率
0.00%
发文量
48
期刊介绍: The journal deals with advances in virology in Russia and abroad. It publishes papers dealing with investigations of viral diseases of man, animals and plants, the results of experimental research on different problems of general and special virology. The journal publishes materials are which promote introduction into practice of the achievements of the virological science in the eradication and incidence reduction of infectious diseases, as well as their diagnosis, treatment and prevention. The reader will find a description of new methods of investigation, new apparatus and devices.
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