A programmable magnetic digital microfluidic platform integrated with electrochemical detection system.

IF 7.3 1区 工程技术 Q1 INSTRUMENTS & INSTRUMENTATION
Yong Zhao, Shuyue Jiang, Gaozhe Cai, Lihua Wang, Jianlong Zhao, Shilun Feng
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引用次数: 0

Abstract

Digital microfluidic (DMF) technology is widely used in bioanalysis and chemical reactions due to its accuracy and flexibility in manipulating droplets. However, most DMF systems usually rely on complex electrode fabrication and high driving voltages. Sensor integration in DMF systems is also quite rare. In this study, a programmable magnetic digital microfluidic (PMDMF) platform integrated with electrochemical detection system was proposed. It enables non-contact, flexible droplet manipulation without complex processes and high voltages, meeting the requirements of automated electrochemical detection. The platform includes a magnetic control system, a microfluidic chip, and an electrochemical detection system. The magnetic control system consists of a microcoil array circuit board, a N52 permanent magnet, and an Arduino control module. N52 magnets generate localized magnetic fields to drive droplet movement, while the Arduino module enables programmable control for precise manipulation. The maximum average velocity of the droplet is about 3.9 cm/s. The microfluidic chip was fabricated using 3D printing and the superhydrophobic surface of chip was fabricated by spray coating. The electrochemical detection system consists of the MoS2@CeO2/PVA working electrode, Ag/AgCl reference electrode, and carbon counter electrode. To evaluate the practical value of the integrated platform, glucose in sweat was automatically and accurately detected. The proposed platform has a wide linear detection range (0.01-0.25 mM), a lower LOD (6.5 μM), a superior sensitivity (7833.54 μA·mM-1·cm-2), and excellent recovery rate (88.1-113.5%). It has an extensive potential for future application in the fields of medical diagnostics and point-of-care testing.

一种集成电化学检测系统的可编程磁数字微流控平台。
数字微流控(DMF)技术以其精确、灵活的微滴操纵技术在生物分析和化学反应中得到了广泛的应用。然而,大多数DMF系统通常依赖于复杂的电极制造和高驱动电压。传感器集成在DMF系统也是相当罕见的。本文提出了一种集成电化学检测系统的可编程磁数字微流控(PMDMF)平台。它可以实现非接触,灵活的液滴操作,不需要复杂的过程和高电压,满足自动化电化学检测的要求。该平台包括一个磁控制系统、一个微流控芯片和一个电化学检测系统。磁控系统由微线圈阵列电路板、N52永磁体和Arduino控制模块组成。N52磁体产生局部磁场来驱动液滴运动,而Arduino模块实现可编程控制,以实现精确操作。液滴的最大平均速度约为3.9 cm/s。采用3D打印技术制备了微流控芯片,并采用喷涂技术制备了芯片的超疏水表面。电化学检测系统由MoS2@CeO2/PVA工作电极、Ag/AgCl参比电极和碳对电极组成。为了评估该集成平台的实用价值,对汗液中的葡萄糖进行了自动准确的检测。该平台线性检测范围宽(0.01 ~ 0.25 mM),检出限低(6.5 μM),灵敏度高(7833.54 μA·mM-1·cm-2),回收率高(88.1 ~ 113.5%)。它在医疗诊断和即时检测领域具有广泛的应用潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Microsystems & Nanoengineering
Microsystems & Nanoengineering Materials Science-Materials Science (miscellaneous)
CiteScore
12.00
自引率
3.80%
发文量
123
审稿时长
20 weeks
期刊介绍: Microsystems & Nanoengineering is a comprehensive online journal that focuses on the field of Micro and Nano Electro Mechanical Systems (MEMS and NEMS). It provides a platform for researchers to share their original research findings and review articles in this area. The journal covers a wide range of topics, from fundamental research to practical applications. Published by Springer Nature, in collaboration with the Aerospace Information Research Institute, Chinese Academy of Sciences, and with the support of the State Key Laboratory of Transducer Technology, it is an esteemed publication in the field. As an open access journal, it offers free access to its content, allowing readers from around the world to benefit from the latest developments in MEMS and NEMS.
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