Characterization of excitatory synaptic transmission in the retrosplenial cortex of adult mice.

IF 2.8 3区医学 Q2 NEUROSCIENCES

Molecular Pain Pub Date : 2025-01-01 Epub Date: 2025-05-03 DOI:10.1177/17448069251335500

Jinjin Wan, Yujie Ma, Xuanying Chen, Wucheng Tao, Shun Hao, Wujun Geng, Yili Wu, Min Zhuo

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Abstract

The retrosplenial cortex (RSC) plays an important role in navigation, memory and pain. However, there are few studies on excitatory synaptic transmission in the RSC. Here, we used a multi-electrode array recording system (MED64) to study the characteristics of excitatory synaptic transmission in the RSC and the contribution of different types of voltage-gated Ca²⁺ channels (VGCCs) in excitatory synaptic transmission. We found that glutamate is the major excitatory transmitter for RSC, and postsynaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptors contribute to excitatory synaptic transmission. We also found that the N-type calcium channel blocker ω-conotoxin GVIA (ω-Ctx GVIA) had an inhibitory effect on basal synaptic transmission. The inhibitory effect was not consistent across channels, suggesting the actions effect of N-type VGCCs in RSC was inhomogeneous in spatial distribution. Our findings provide strong evidence that excitatory synaptic transmission in the RSC is mainly mediated by AMPA receptors and that N-type VGCCs mediate fast synaptic transmission in the RSC of adult mice.

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成年小鼠脾后皮层兴奋性突触传递的表征。

脾后皮层（RSC）在导航、记忆和疼痛中起着重要作用。然而，关于RSC兴奋性突触传递的研究很少。本研究利用多电极阵列记录系统（MED64）研究了RSC中兴奋性突触传递的特征，以及不同类型的电压门控Ca2+通道（VGCCs）在兴奋性突触传递中的作用。我们发现谷氨酸是RSC的主要兴奋性递质，突触后α -氨基-3-羟基-5-甲基-4-异氧唑丙酸（AMPA）受体参与兴奋性突触传递。我们还发现n型钙通道阻滞剂ω- concontoxin GVIA （ω-Ctx GVIA）对基底突触传递有抑制作用。不同通道的抑制作用并不一致，表明这些兴奋性突触对突触前n型VGCCs的贡献是不均匀的。我们的研究结果有力地证明了RSC中的兴奋性突触传递主要是由AMPA受体介导的，而n型VGCCs介导了成年小鼠RSC中的快速突触传递。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Pain 医学-神经科学

CiteScore

5.60

自引率

3.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Molecular Pain is a peer-reviewed, open access journal that considers manuscripts in pain research at the cellular, subcellular and molecular levels. Molecular Pain provides a forum for molecular pain scientists to communicate their research findings in a targeted manner to others in this important and growing field.