Assessment of Heat Stroke-Induced Brain Injury: A Preclinical Study with a Rat Model Using ¹⁸F-FDG Brain PET.

IF 2.5 4区医学 Q2 RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING

Molecular Imaging and Biology Pub Date : 2025-06-01 Epub Date: 2025-04-15 DOI:10.1007/s11307-025-02008-1

Daehee Kim, Hye Won Lee, Byung Seok Moon, Sun Mi Park, Ji Eun Lee, Bom Sahn Kim, Woon Jeong Lee, Hai-Jeon Yoon

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引用次数: 0

Abstract

Purpose: Heat stroke is the most serious heat-related illness and is recognized as a worldwide public concern as global temperatures continue to rise. Although the clinical neurological complications of heat stroke are relatively well described, a limited number of studies exist that document imaging findings. Therefore, in this preclinical study, we aimed to identify the imaging findings of ¹⁸F-FDG brain PET following heat stroke and elucidate the utility of FDG PET in the evaluation of heat stroke-induced brain injury.

Methods: Heat stroke was induced in Sprague Dawley rats by placing them in a hot and humid chamber maintained without food and water until they exhibited the heat stroke onset diagnostic criterion. Three hours after the induction ended, ¹⁸F-FDG brain PET images were acquired in 7 controls and 14 rats with heat stroke. Between groups, region-based (standardized uptake values were normalized to the whole brain and SUV of the whole brain (SUV_WB), and voxel-based analyses were performed.

Results: Of the 14 rats with heat stroke, 8 survived, whereas 6 did not. In the region-based and voxel-base analyses, the rats that did not survive showed significantly higher SUVR_HB in the hypothalamus and significantly lower SUVR_HB in several cortical regions than the controls as well as the survived rats. In the region-based analysis, the survived rats showed a significant increase or decrease in SUVR_HB compared to the controls in a few cortical regions. However, no difference was observed in the voxel-based analysis.

Conclusions: The 3-h post-injury PET scan showed a distinctly different regional distribution of ¹⁸F-FDG in the brains of lethally injured heat stroke rats compared to the controls as well as the survived rats. The ¹⁸F-FDG brain PET may have the potential to provide early indicators of catastrophic injury and reflect the early neurological pathophysiology of heat stroke.

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18F-FDG脑PET评估大鼠中暑脑损伤的临床前研究

目的：中暑是最严重的与热有关的疾病，随着全球气温持续上升，中暑已被公认为全球公众关注的问题。尽管中暑的临床神经系统并发症已经得到了较好的描述，但仅有有限数量的研究记录了影像学结果。因此，在本临床前研究中，我们旨在确定18F-FDG脑PET在中暑后的影像学表现，并阐明FDG PET在评估中暑性脑损伤中的应用。方法：将Sprague Dawley大鼠置于闷热潮湿的室内，不给食物和水，直到它们表现出中暑发作的诊断标准。诱导结束3小时后，对7只对照组和14只中暑大鼠进行18F-FDG脑PET成像。在两组之间，将基于区域（标准化）的摄取值归一化为全脑和全脑SUV (SUVWB)，并进行基于体素的分析。结果：14只中暑大鼠中，8只存活，6只死亡。在基于区域和基于体素的分析中，与对照组和存活的大鼠相比，未存活的大鼠下丘脑的SUVRHB显著升高，几个皮质区域的SUVRHB显著降低。在基于区域的分析中，与对照组相比，存活的大鼠在少数皮质区域显示SUVRHB显著增加或减少。然而，在基于体素的分析中没有观察到差异。结论：致死性中暑大鼠损伤后3小时PET扫描显示，18F-FDG在脑内的区域分布与对照组及存活大鼠明显不同。18F-FDG脑PET可能有潜力提供灾难性损伤的早期指标，并反映中暑的早期神经病理生理。

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来源期刊

Molecular Imaging and Biology 医学-核医学

CiteScore

6.90

自引率

3.20%

发文量

审稿时长

3 months

期刊介绍： Molecular Imaging and Biology (MIB) invites original contributions (research articles, review articles, commentaries, etc.) on the utilization of molecular imaging (i.e., nuclear imaging, optical imaging, autoradiography and pathology, MRI, MPI, ultrasound imaging, radiomics/genomics etc.) to investigate questions related to biology and health. The objective of MIB is to provide a forum to the discovery of molecular mechanisms of disease through the use of imaging techniques. We aim to investigate the biological nature of disease in patients and establish new molecular imaging diagnostic and therapy procedures. Some areas that are covered are: Preclinical and clinical imaging of macromolecular targets (e.g., genes, receptors, enzymes) involved in significant biological processes. The design, characterization, and study of new molecular imaging probes and contrast agents for the functional interrogation of macromolecular targets. Development and evaluation of imaging systems including instrumentation, image reconstruction algorithms, image analysis, and display. Development of molecular assay approaches leading to quantification of the biological information obtained in molecular imaging. Study of in vivo animal models of disease for the development of new molecular diagnostics and therapeutics. Extension of in vitro and in vivo discoveries using disease models, into well designed clinical research investigations. Clinical molecular imaging involving clinical investigations, clinical trials and medical management or cost-effectiveness studies.