Uncovering Non-Invasive Biomarkers in Paediatric Severe Acute Asthma Using Targeted Exhaled Breath Analysis.

IF 3.4 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Metabolites Pub Date : 2025-04-03 DOI:10.3390/metabo15040247

Sarah van den Berg, Annabel S Zaat, Isabel F van der Poel, Yoni E van Dijk, Simone Hashimoto, Niels W P Rutjes, Suzanne W J Terheggen-Largo, Bart E van Ewijk, Claudia Gagliani, Fleur L Sondaal, Job B M van Woensel, Anke-Hilse Maitland-van der Zee, Paul Brinkman, Susanne J H Vijverberg, Berber Kapitein

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引用次数: 0

Abstract

Background: Severe acute asthma (SAA) in children can be life-threatening. There has been a significant rise in paediatric intensive care unit (PICU) admissions due to SAA over the past two decades. While asthma is a heterogeneous disease, its underlying pathophysiological pathways remain underexplored. This study aimed to assess the value of non-invasive targeted exhaled breath metabolomics analysis to better characterise SAA.

Methods: Breath samples from 17 children admitted to the PICU with SAA (cases) and 27 children with controlled severe asthma (controls) were analysed using thermal desorption gas chromatography-mass spectrometry (TD-GC-MS).

Results: A targeted volatile organic compound (VOC) analysis identified 25 compounds, of which 16 were shared between groups. Four VOCs were significantly more often present in SAA, and nine VOCs exhibited higher concentrations in SAA. Longitudinal analysis of VOCs from follow-up samples of 10 cases showed no significant temporal differences, reinforcing the reproducibility of identified biomarkers.

Conclusions: This study exemplifies the potential of exhaled breath analysis to provide insights into the molecular background of SAA. Breath metabolomics may enable early recognition of severe asthma attacks and preventive therapeutic interventions in children with severe asthma.

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利用有针对性的呼气分析发现儿童严重急性哮喘的非侵入性生物标志物。

背景：儿童严重急性哮喘（SAA）可危及生命。在过去的二十年中，由于SAA，儿科重症监护病房（PICU）入院人数显著上升。虽然哮喘是一种异质性疾病，但其潜在的病理生理途径仍未得到充分探索。本研究旨在评估非侵入性呼气代谢组学分析的价值，以更好地表征SAA。方法：采用热解吸气相色谱-质谱联用（TD-GC-MS）对PICU收治的17例SAA患儿（病例）和27例对照重症哮喘患儿（对照）的呼气样本进行分析。结果：有针对性的挥发性有机化合物（VOC）分析鉴定出25种化合物，其中16种化合物在两组之间共享。4种挥发性有机化合物在SAA中出现的频率显著高于SAA， 9种挥发性有机化合物在SAA中的浓度更高。对10例随访样本的挥发性有机化合物的纵向分析没有发现显著的时间差异，加强了所鉴定生物标志物的可重复性。结论：本研究体现了呼气分析在SAA分子背景研究中的潜力。呼吸代谢组学可能使严重哮喘发作的早期识别和严重哮喘儿童的预防性治疗干预成为可能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Metabolites Biochemistry, Genetics and Molecular Biology-Molecular Biology

CiteScore

5.70

自引率

7.30%

发文量

1070

审稿时长

17.17 days

期刊介绍： Metabolites (ISSN 2218-1989) is an international, peer-reviewed open access journal of metabolism and metabolomics. Metabolites publishes original research articles and review articles in all molecular aspects of metabolism relevant to the fields of metabolomics, metabolic biochemistry, computational and systems biology, biotechnology and medicine, with a particular focus on the biological roles of metabolites and small molecule biomarkers. Metabolites encourages scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on article length. Sufficient experimental details must be provided to enable the results to be accurately reproduced. Electronic material representing additional figures, materials and methods explanation, or supporting results and evidence can be submitted with the main manuscript as supplementary material.