Evaluation of canine epidermis and keratinocytoids (keratinocytic organoids) by transmission electron microscopy.

IF 1.4 3区农林科学 Q3 DERMATOLOGY

Veterinary dermatology Pub Date : 2025-10-01 Epub Date: 2025-05-14 DOI:10.1111/vde.13356

Clinson C Lui, Dominique J Wiener, Katia R Groch, Lauren W Stranahan, Christina R Heard, Aníbal G Armién

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Abstract

Background: Skin disease is a common complaint in veterinary medicine. Current models often use live animals. Epidermal organoids (keratinocytoids) are 3D miniature organs created in culture from single epidermal keratinocytes. These keratinocytoids can be used as an alternative to live animal studies to investigate epidermal structures and skin diseases.

Hypothesis/objective: This study's objective was to compare ultrastructural morphological features of canine keratinocytoids to those features of normal epidermis.

Results: Skin and organoids had morphologically similar components, including tight junctions, desmosomes, lamellar bodies and keratin filaments. These structures were similar in both morphology and distribution. Keratinocytes in the organoids had larger and more distinct keratohyalin granules than epidermal keratinocytes and contained small amounts of glycogen. Keratinocytes from the skin showed no glycogen accumulation.

Conclusions and clinical relevance: Canine keratinocytoids are a useful model to study canine epidermal disease from a light microscopy and ultrastructural standpoint.

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犬表皮和角化细胞样（角化细胞类器官）的透射电镜评价。

背景：皮肤病是兽医学中一种常见的疾病。目前的模型通常使用活体动物。表皮类器官（类角质细胞）是由单个表皮角质形成细胞培养而成的三维微型器官。这些角化细胞样物可以作为活体动物研究的替代方法来研究表皮结构和皮肤病。假设/目的：本研究的目的是比较犬角化细胞样细胞与正常表皮的超微结构形态特征。结果：皮肤和类器官在形态上具有相似的成分，包括紧密连接、桥粒、板层体和角蛋白丝。这些结构在形态和分布上都相似。类器官中的角质形成细胞比表皮角质形成细胞有更大、更明显的角质透明素颗粒，并含有少量的糖原。皮肤角质形成细胞未见糖原积聚。结论和临床意义：犬角化细胞样物是研究犬表皮疾病的一个有用的模型，从光镜和超微结构的角度来看。

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来源期刊

Veterinary dermatology 农林科学-兽医学

CiteScore

3.20

自引率

21.40%

发文量

审稿时长

12-24 weeks

期刊介绍： Veterinary Dermatology is a bi-monthly, peer-reviewed, international journal which publishes papers on all aspects of the skin of mammals, birds, reptiles, amphibians and fish. Scientific research papers, clinical case reports and reviews covering the following aspects of dermatology will be considered for publication: -Skin structure (anatomy, histology, ultrastructure) -Skin function (physiology, biochemistry, pharmacology, immunology, genetics) -Skin microbiology and parasitology -Dermatopathology -Pathogenesis, diagnosis and treatment of skin diseases -New disease entities