Natural variations in the promoter of ZmDeSI2 encoding a deSUMOylating isopeptidase controls kernel methionine content in maize.

IF 17.1 1区 生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Molecular Plant Pub Date : 2025-05-05 Epub Date: 2025-04-22 DOI:10.1016/j.molp.2025.04.008
Xin Lu, Yuhong Lei, Zhennan Xu, Zixiang Cheng, Meng Liu, Yuxin Tai, Xiaohua Han, Zhuanfang Hao, Mingshun Li, Degui Zhang, Hongjun Yong, Jienan Han, Zhenhua Wang, Wen-Xue Li, Jianfeng Weng, Zhiqiang Zhou, Xinhai Li
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引用次数: 0

Abstract

Improving the methionine (Met) content in maize kernels is of key importance to the animal feed industry; however, the genetic and molecular mechanisms governing maize kernel Met content remain largely unexplored. In this study, we leveraged a panel consisting of 348 diverse inbred maize lines to explore the genetic and molecular mechanisms that control kernel Met levels. A genome-wide association study followed by transcriptomic analysis identified the deSUMOylating isopeptidase gene ZmDeSI2. Further biochemical experiments revealed that ZmDeSI2 directly reduces the SUMOylation and accumulation of the sulfite reductase ZmSIR, thereby repressing Met accumulation. Natural variants in the ZmDeSI2 promoter region were found to serve as key determinants of the expression of this gene, predominantly due to the absence or presence of a ZmWRKY105 transcription factor binding site. The elite ZmDeSI2Hap2 haplotype without this binding site in the ZmDeSI2 promoter was associated with a 1.36-fold increase in Met levels in the kernels of modified near-isogenic lines generated through marker-assisted breeding. Taken together, these results provide new insights into the molecular processes that control Met biosynthesis, highlighting an elite natural variant suitable for application in maize breeding for Met biofortification.

编码脱硫异肽酶的ZmDeSI2启动子的自然变异控制玉米籽粒蛋氨酸含量。
提高玉米籽粒中蛋氨酸(Met)的含量对动物饲料工业具有重要意义。然而,控制玉米籽粒Met含量的遗传和分子机制在很大程度上仍未被探索。在这项研究中,我们利用一个由348个不同自交系组成的小组来探索控制籽粒Met水平的遗传和分子机制。一项全基因组关联研究和转录组学分析鉴定了去苏甲基异肽酶基因ZmDeSI2。进一步的生化实验表明,ZmDeSI2直接降低亚硫酸盐还原酶ZmSIR的sumo化和积累,从而抑制Met的积累。ZmDeSI2启动子区域的自然变异被发现是该基因表达的关键决定因素,主要是由于ZmWRKY105转录因子结合位点的缺失或存在。在ZmDeSI2启动子中没有这个结合位点的精英ZmDeSI2Hap2单倍型与通过标记辅助育种产生的修饰近等基因系的籽粒Met水平增加1.36倍相关。综上所述,这些结果为控制Met生物合成的分子过程提供了新的见解,突出了适合在玉米育种中应用Met生物强化的精英自然变体。
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来源期刊
Molecular Plant
Molecular Plant 植物科学-生化与分子生物学
CiteScore
37.60
自引率
2.20%
发文量
1784
审稿时长
1 months
期刊介绍: Molecular Plant is dedicated to serving the plant science community by publishing novel and exciting findings with high significance in plant biology. The journal focuses broadly on cellular biology, physiology, biochemistry, molecular biology, genetics, development, plant-microbe interaction, genomics, bioinformatics, and molecular evolution. Molecular Plant publishes original research articles, reviews, Correspondence, and Spotlights on the most important developments in plant biology.
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