Anjali Verma, Rakhi Singh, Shoeb Ahmed, Rahul Kumar, Shailendra Sharma, H S Dhaliwal, H S Balyan, P K Gupta
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引用次数: 0
Abstract
Wheat serves as the primary source of staple food for the global human population, thus also making it a significant portion of the calorie intake in our daily vegetarian diets. However, in most of the improved wheat cultivars used for food, the grain is deficient in iron (Fe) and zinc (Zn). Therefore, biofortification involving improvement of grain Fe and Zn has become an important area in the current wheat breeding programmes. For this purpose, efforts have been made to develop alien substitution lines and utilize them for transfer of desirable alien genes to improved wheat cultivars. In the present study, two such genotypes in the background of improved cultivar PBW343LrYr were utilized for pyramiding of the following six desirable genes for enrichment of grain Fe and Zn: IRT2, MTP3, IREG, FRO7, YSL15 and NAS2. A forward breeding strategy, involving crossing of the two genotypes followed by inbreeding was used. Marker-assisted selection (MAS) of the genes of interest associated with grain Fe/Zn and plant type was used following selfing of F1 hybrids. The grains of F6 lines that were derived in this programmes were rich in both Fe and Zn contents in the grain. Among the six best derived lines, the values of improved contents of grain Fe ranged from 47.3 to 60.4 ppm and that of Zn ranged from 39.35 to 47.85 ppm. There was no yield penalty in these improved lines, such that the yield was either equal or better than the checks used in field trials.
Supplementary information: The online version contains supplementary material available at 10.1007/s11032-025-01566-0.
期刊介绍：
Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer.
All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others.
Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards.
Molecular Breeding core areas:
Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.
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