The Potato StNAC2-StSABP2 Module Enhanced Resistance to Phytophthora infestans Through Activating the Salicylic Acid Pathway.

IF 4.8 1区 农林科学 Q1 PLANT SCIENCES
Jie Yan, Qian Li, Deying Geng, Zheng Wang, Dongmei Zhao, Dai Zhang, Jinhui Wang, Yang Pan, Jiehua Zhu, Zhihui Yang
{"title":"The Potato StNAC2-StSABP2 Module Enhanced Resistance to Phytophthora infestans Through Activating the Salicylic Acid Pathway.","authors":"Jie Yan, Qian Li, Deying Geng, Zheng Wang, Dongmei Zhao, Dai Zhang, Jinhui Wang, Yang Pan, Jiehua Zhu, Zhihui Yang","doi":"10.1111/mpp.70081","DOIUrl":null,"url":null,"abstract":"<p><p>Potato late blight is an important disease in potato production, which causes serious damage. Salicylic acid (SA) is a plant hormone involved in the regulation of potato (Solanum tuberosum) resistance to Phytophthora infestans. In this study, it was found that exogenous methyl salicylate (MeSA) treatment could significantly enhance the resistance of potato to P. infestans. RNA-seq results confirmed that SA was important for potato resistance to P. infestans. Salicylic acid binding protein 2 (SABP2) is a member of α/β hydrolase family, which can convert MeSA into SA to regulate the steady state of SA in plants. StSABP2 protein was obtained through prokaryotic expression, and enzymatic analysis in vitro confirmed that StSABP2 could transform MeSA into SA. In order to explore the function of StSABP2 in the process of plant resistance to P. infestans, we carried out virus-mediated gene silencing of StSABP2 in potato and transiently expressed StSABP2 in tobacco. The results showed that StSABP2 positively regulated plant resistance to P. infestans, and this process was achieved by mediating the transcription of SA signal and defence-related genes. Then we screened for the upstream regulator of StSABP2. The results of double luciferase and yeast one-hybrid analysis showed that StNAC2 could activate the transcription of StSABP2. The StNAC2-StSABP2 module regulated potato resistance to P. infestans by positively mediating the SA pathway. This study provides a new idea for improving host resistance to potato late blight by regulating the SA signal in potato and provides germplasm resources for potato resistance breeding.</p>","PeriodicalId":18763,"journal":{"name":"Molecular plant pathology","volume":"26 5","pages":"e70081"},"PeriodicalIF":4.8000,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12040442/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Molecular plant pathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1111/mpp.70081","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Potato late blight is an important disease in potato production, which causes serious damage. Salicylic acid (SA) is a plant hormone involved in the regulation of potato (Solanum tuberosum) resistance to Phytophthora infestans. In this study, it was found that exogenous methyl salicylate (MeSA) treatment could significantly enhance the resistance of potato to P. infestans. RNA-seq results confirmed that SA was important for potato resistance to P. infestans. Salicylic acid binding protein 2 (SABP2) is a member of α/β hydrolase family, which can convert MeSA into SA to regulate the steady state of SA in plants. StSABP2 protein was obtained through prokaryotic expression, and enzymatic analysis in vitro confirmed that StSABP2 could transform MeSA into SA. In order to explore the function of StSABP2 in the process of plant resistance to P. infestans, we carried out virus-mediated gene silencing of StSABP2 in potato and transiently expressed StSABP2 in tobacco. The results showed that StSABP2 positively regulated plant resistance to P. infestans, and this process was achieved by mediating the transcription of SA signal and defence-related genes. Then we screened for the upstream regulator of StSABP2. The results of double luciferase and yeast one-hybrid analysis showed that StNAC2 could activate the transcription of StSABP2. The StNAC2-StSABP2 module regulated potato resistance to P. infestans by positively mediating the SA pathway. This study provides a new idea for improving host resistance to potato late blight by regulating the SA signal in potato and provides germplasm resources for potato resistance breeding.

马铃薯StNAC2-StSABP2模块通过激活水杨酸途径增强对疫霉菌的抗性
马铃薯晚疫病是马铃薯生产中的重要病害,危害严重。水杨酸(Salicylic acid, SA)是一种参与调节马铃薯(Solanum tuberosum)对疫霉抗性的植物激素。本研究发现外源水杨酸甲酯(MeSA)处理能显著增强马铃薯对病原菌的抗性。RNA-seq结果证实,SA在马铃薯对病原菌的抗性中起重要作用。水杨酸结合蛋白2 (Salicylic acid binding protein 2, SABP2)是α/β水解酶家族成员,可将MeSA转化为SA,调控植物SA稳态。通过原核表达获得StSABP2蛋白,体外酶促分析证实StSABP2能将MeSA转化为SA。为了探究StSABP2在植物抗鼠疫杆菌过程中的作用,我们在马铃薯中进行了病毒介导的StSABP2基因沉默,并在烟草中短暂表达了StSABP2。结果表明,StSABP2通过介导SA信号和防御相关基因的转录,正调控了植物对病原菌的抗性。然后我们筛选StSABP2的上游调控因子。双荧光素酶和酵母单杂交分析结果表明,StNAC2可以激活StSABP2的转录。StNAC2-StSABP2模块通过正向介导SA途径调控马铃薯对病原菌的抗性。本研究为通过调控马铃薯SA信号提高寄主对马铃薯晚疫病的抗性提供了新思路,并为马铃薯抗病育种提供了种质资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Molecular plant pathology
Molecular plant pathology 生物-植物科学
CiteScore
9.40
自引率
4.10%
发文量
120
审稿时长
6-12 weeks
期刊介绍: Molecular Plant Pathology is now an open access journal. Authors pay an article processing charge to publish in the journal and all articles will be freely available to anyone. BSPP members will be granted a 20% discount on article charges. The Editorial focus and policy of the journal has not be changed and the editorial team will continue to apply the same rigorous standards of peer review and acceptance criteria.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信