Spectrum of AGT (M235T) rs699 and AGTR1 (A1166C) rs5186 gene variants and its association with coronary artery disease in Pakistani patients.

IF 1.2 4区 医学 Q2 MEDICINE, GENERAL & INTERNAL
Syed Tousif Ahmed, Muhammad Israr Nasir, Kanwal Fatima Amir, Pirzada Qasim Raza Siddiqui
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Abstract

Background and objective: Coronary artery disease (CAD) is a multifaceted ailment influenced by genetic and acquired factors. In this study we tried to determine the association of CAD with polymorphisms in renin-angiotensin-aldosterone system (RAAS) genes AGT(M235T) rs699 and AGTRI(A1166C) rs5186.

Method: This case-control study was conducted at Ziauddin University and National Institute of Cardiovascular Diseases Karachi from January, 2019 to June, 2020. It included 239 participants between 30-70years from both genders via convenient sampling. The participants were divided into two groups of 160 controls and 79 angiographically diagnosed CAD patients. Genotyping of AGT(M235T) and AGTRI(A1166C) was investigated by the allele-specific polymerase chain reaction (AS-PCR). Statistical analysis was done using SPSS Version-22. Independent sample t-test was applied for comparison of quantitative variables. The AGT(M235T) and AGRT1(A1166) genes were compared by Chi- square test.

Results: There was no significant association found between CAD and AGT(M235T) gene variants CC, CT and TT (p=0.3; p=0.1; p=0.6 respectively). AGTRI(A1166) of AA and CC variety showed significant association with CAD(p<0.001), while its AC variant showed no significant association with the disease. The odds of CC of AGRT1(A1166C) having CAD were 14 times more, whereas having CAD with AA of AGRT1(A1166C) were 70% less.

Conclusion: Individuals with CC polymorphisms of AGTRI(A1166) gene are 14 times more likely to develop CAD, whereas those with AA variation are less likely to develop the disease. AC variation of the AGTRI(A1166) gene along with all variations of the AGT(M235T) gene were not associated with development of CAD.

巴基斯坦患者AGT (M235T) rs699和AGTR1 (A1166C) rs5186基因变异谱及其与冠状动脉疾病的关系
背景与目的:冠状动脉疾病(CAD)是一种受遗传和后天因素影响的多方面疾病。在这项研究中,我们试图确定CAD与肾素-血管紧张素-醛固酮系统(RAAS)基因AGT(M235T) rs699和AGTRI(A1166C) rs5186多态性的关系。方法:病例对照研究于2019年1月- 2020年6月在齐亚乌丁大学和卡拉奇国立心血管疾病研究所进行。通过方便的抽样,该研究包括了239名年龄在30-70岁之间的男女参与者。参与者被分为两组,160名对照组和79名经血管造影诊断为CAD的患者。采用等位基因特异性聚合酶链反应(AS-PCR)对AGT(M235T)和AGTRI(A1166C)进行基因分型研究。采用SPSS Version-22进行统计分析。定量变量比较采用独立样本t检验。AGT(M235T)基因与agt1 (A1166)基因采用卡方检验进行比较。结果:冠心病与AGT(M235T)基因变异CC、CT、TT无显著相关性(p=0.3;p = 0.1;p = 0.6)。短句来源结论:AGTRI(A1166)基因CC多态性个体发生冠心病的可能性是AGTRI(A1166) CC多态性个体的14倍,而AA变异个体发生冠心病的可能性较低。AGTRI(A1166)基因的AC变异以及AGT(M235T)基因的所有变异与CAD的发展无关。
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来源期刊
Pakistan Journal of Medical Sciences
Pakistan Journal of Medical Sciences 医学-医学:内科
CiteScore
4.10
自引率
9.10%
发文量
363
审稿时长
3-6 weeks
期刊介绍: It is a peer reviewed medical journal published regularly since 1984. It was previously known as quarterly "SPECIALIST" till December 31st 1999. It publishes original research articles, review articles, current practices, short communications & case reports. It attracts manuscripts not only from within Pakistan but also from over fifty countries from abroad. Copies of PJMS are sent to all the import medical libraries all over Pakistan and overseas particularly in South East Asia and Asia Pacific besides WHO EMRO Region countries. Eminent members of the medical profession at home and abroad regularly contribute their write-ups, manuscripts in our publications. We pursue an independent editorial policy, which allows an opportunity to the healthcare professionals to express their views without any fear or favour. That is why many opinion makers among the medical and pharmaceutical profession use this publication to communicate their viewpoint.
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