Levels of IL-1β, MMP-8, and MMP-9 in the Saliva of Subjects With Periodontitis: A Systematic Review and Meta-Analysis

IF 2.6 4区医学 Q2 MEDICAL LABORATORY TECHNOLOGY

Journal of Clinical Laboratory Analysis Pub Date : 2025-04-27 DOI:10.1002/jcla.70040

Mario Alberto Alarcón-Sánchez, Ruth Rodríguez-Montaño, Seyed Ali Mosaddad, Artak Heboyan

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引用次数: 0

Abstract

Background and Objective

Proinflammatory cytokines and enzymes responsible for tissue destruction are important in the development of periodontitis. This study compared salivary concentrations of interleukin-1 beta (IL-1β), matrix metalloproteinases (MMP-8), and (MMP-9) in individuals with and without periodontitis to evaluate their diagnostic utility as potential biomarkers.

Materials and Methods

A comprehensive search was performed across PubMed, Scopus, ScienceDirect, and Google Scholar, supplemented by manual searches in relevant journals up to January 2024. Eligibility criteria focused on human studies with defined diagnostic criteria for periodontitis and saliva samples analyzed for IL-1β, MMP-8, and MMP-9. Data were extracted to compare salivary levels of these markers between periodontitis patients and healthy controls. The Joanna Briggs Institute tool was used to evaluate the risk of bias and quality of the included studies. Statistical analysis employed a random effects model to calculate standardized mean differences and assess heterogeneity and publication bias.

Results

The search yielded 122 articles, with 27 meeting the inclusion criteria. Fifteen percent of these studies presented a moderate risk of bias, while the remaining 85% exhibited a low risk of bias. The meta-analyses indicated significantly higher levels of IL-1β, MMP-8, and MMP-9 in the saliva of subjects with periodontitis compared to healthy individuals: IL-1β: Standardized Mean Difference (SMD) = 163.29 (95% CI = 104.64–221.95), p < 0.001; MMP-8: SMD = 282.22 (95% CI = 209.68–354.77), p < 0.001; MMP-9: SMD = 311.85 (95% CI = 179.64–444.05), p < 0.001.

Conclusion

Elevated salivary levels of IL-1β, MMP-8, and MMP-9 are linked to periodontitis.

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牙周炎患者唾液中IL-1β、MMP-8和MMP-9水平的系统回顾和荟萃分析

背景和目的：负责组织破坏的促炎细胞因子和酶在牙周炎的发展中很重要。本研究比较了牙周炎患者和非牙周炎患者唾液中白细胞介素-1β (IL-1β)、基质金属蛋白酶（MMP-8）和（MMP-9）的浓度，以评估其作为潜在生物标志物的诊断价值。材料和方法：综合检索PubMed、Scopus、ScienceDirect和b谷歌Scholar，并辅以人工检索截止到2024年1月的相关期刊。资格标准主要集中在具有牙周炎明确诊断标准的人类研究，并分析唾液样本中的IL-1β、MMP-8和MMP-9。提取数据来比较牙周炎患者和健康对照者唾液中这些标志物的水平。乔安娜布里格斯研究所的工具被用来评估纳入研究的偏倚风险和质量。统计分析采用随机效应模型计算标准化平均差异，评估异质性和发表偏倚。结果：检索到122篇文献，其中27篇符合纳入标准。这些研究中有15%表现出中等偏倚风险，而其余85%表现出低偏倚风险。荟萃分析显示，牙周炎患者唾液中IL-1β、MMP-8和MMP-9的水平明显高于健康人群：IL-1β：标准化平均差异(SMD) = 163.29 (95% CI = 104.64-221.95)， p结论：唾液中IL-1β、MMP-8和MMP-9水平升高与牙周炎有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Clinical Laboratory Analysis 医学-医学实验技术

CiteScore

5.60

自引率

7.40%

发文量

584

审稿时长

6-12 weeks

期刊介绍： Journal of Clinical Laboratory Analysis publishes original articles on newly developing modes of technology and laboratory assays, with emphasis on their application in current and future clinical laboratory testing. This includes reports from the following fields: immunochemistry and toxicology, hematology and hematopathology, immunopathology, molecular diagnostics, microbiology, genetic testing, immunohematology, and clinical chemistry.