Effects of Nonablative Er-YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study

IF 2.2 3区医学 Q2 DERMATOLOGY

Lasers in Surgery and Medicine Pub Date : 2025-04-24 DOI:10.1002/lsm.70020

Cemal Tamer Erel, Duygu Neccar, Ipek Betul Ozcivit Erkan, Neslihan Gokmen Inan, Elif Guzel Meydanli

{"title":"Effects of Nonablative Er-YAG Laser on Human Endometrial Stromal Cells (hESCs): A Pilot Study","authors":"Cemal Tamer Erel, Duygu Neccar, Ipek Betul Ozcivit Erkan, Neslihan Gokmen Inan, Elif Guzel Meydanli","doi":"10.1002/lsm.70020","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Objectives</h3>\n \n <p>To investigate the thermo-chemical effects of nonablative Er:YAG laser on human endometrial stromal cells (hESCs).</p>\n </section>\n \n <section>\n \n <h3> Materials and Methods</h3>\n \n <p>In this laboratory based study, for the hESCs cultures, endometrial tissue biopsy samples were obtained from three fertile women in the late proliferative phase. In this laboratory-based study, seven experimental groups were created: Estradiol (E<sub>2</sub>) group, E<sub>2</sub> + Sham (S) group, E<sub>2</sub> + P<sub>4</sub> group, E<sub>2</sub> + Laser (L) group, E<sub>2</sub> + P<sub>4</sub> + L group, hESC group and hESC+L group. Cell cultures reaching confluence on the matrigel-coated petri dishes in all groups were incubated for 12, 24, 48, and 72 h after laser irradiation, respectively. Nonablative Er:YAG laser irradiation was performed to the hESCs culture. The main outcome of this study involves assessing the alterations in MMP-2, TNF-α, IL-6, VEGF-A, and IGFBP-1 levels within conditioned media of hESC cultures subsequent to the Er:YAG laser irradiation.</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>Median MMP-2 levels significantly differed between groups at the 12th, 24th, 48th, and 72nd hour (<i>p</i> = 0.012, <i>p</i> = 0.045, <i>p</i> = 0.021, and <i>p</i> = 0.032, respectively). All media of hESCs irradiated with Er-YAG laser had higher median MMP-2 values. At the 48th hour, the groups showed significant differences in terms of median TNF-α levels (<i>p</i> = 0.029), which were lower in the groups that received Er-YAG laser. At the 12th hour, median IL-6 levels differed significantly between the groups (<i>p</i> = 0.011), being higher in the groups that received Er-YAG laser. At the 72nd hour, lower median VEGF-A values were observed for the groups that received Er-YAG laser (<i>p</i> = 0.021). A statistically significant difference existed in terms of median IGFBP-1 levels between the groups at the 24th hour, with higher levels in the groups which received Er-YAG laser (<i>p</i> = 0.041).</p>\n </section>\n \n <section>\n \n <h3> Conclusion</h3>\n \n <p>Our findings demonstrated that the non-ablative Er-YAG laser induces tissue remodeling, modulates immune responses by favoring Th2 cell activity and suppresses Th1 cell activity in hESCs. Additionally, it exerts favorable impact on decidualization.</p>\n </section>\n </div>","PeriodicalId":17961,"journal":{"name":"Lasers in Surgery and Medicine","volume":"57 5","pages":"415-425"},"PeriodicalIF":2.2000,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/lsm.70020","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Lasers in Surgery and Medicine","FirstCategoryId":"3","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/lsm.70020","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"DERMATOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Objectives

To investigate the thermo-chemical effects of nonablative Er:YAG laser on human endometrial stromal cells (hESCs).

Materials and Methods

In this laboratory based study, for the hESCs cultures, endometrial tissue biopsy samples were obtained from three fertile women in the late proliferative phase. In this laboratory-based study, seven experimental groups were created: Estradiol (E₂) group, E₂ + Sham (S) group, E₂ + P₄ group, E₂ + Laser (L) group, E₂ + P₄ + L group, hESC group and hESC+L group. Cell cultures reaching confluence on the matrigel-coated petri dishes in all groups were incubated for 12, 24, 48, and 72 h after laser irradiation, respectively. Nonablative Er:YAG laser irradiation was performed to the hESCs culture. The main outcome of this study involves assessing the alterations in MMP-2, TNF-α, IL-6, VEGF-A, and IGFBP-1 levels within conditioned media of hESC cultures subsequent to the Er:YAG laser irradiation.

Results

Median MMP-2 levels significantly differed between groups at the 12th, 24th, 48th, and 72nd hour (p = 0.012, p = 0.045, p = 0.021, and p = 0.032, respectively). All media of hESCs irradiated with Er-YAG laser had higher median MMP-2 values. At the 48th hour, the groups showed significant differences in terms of median TNF-α levels (p = 0.029), which were lower in the groups that received Er-YAG laser. At the 12th hour, median IL-6 levels differed significantly between the groups (p = 0.011), being higher in the groups that received Er-YAG laser. At the 72nd hour, lower median VEGF-A values were observed for the groups that received Er-YAG laser (p = 0.021). A statistically significant difference existed in terms of median IGFBP-1 levels between the groups at the 24th hour, with higher levels in the groups which received Er-YAG laser (p = 0.041).

Conclusion

Our findings demonstrated that the non-ablative Er-YAG laser induces tissue remodeling, modulates immune responses by favoring Th2 cell activity and suppresses Th1 cell activity in hESCs. Additionally, it exerts favorable impact on decidualization.

Abstract Image

查看原文本刊更多论文

非烧蚀性Er-YAG激光对人子宫内膜基质细胞（hESCs）影响的初步研究。

目的：探讨非烧蚀性Er:YAG激光对人子宫内膜基质细胞（hESCs）的热化学作用。材料和方法：在本实验室基础上的研究中，hESCs培养，子宫内膜组织活检样本来自三名晚期增殖期的育龄妇女。本实验以实验室为基础，设Estradiol （E2）组、E2 + Sham (S)组、E2 + P4组、E2 + Laser (L)组、E2 + P4 +L组、hESC组和hESC+L组。激光照射后，各组细胞在基质包被培养皿上汇合，分别孵育12、24、48和72 h。采用Er:YAG非烧蚀激光照射hESCs培养。本研究的主要结果包括评估Er:YAG激光照射后hESC培养条件培养基中MMP-2、TNF-α、IL-6、VEGF-A和IGFBP-1水平的变化。结果：12、24、48、72小时组间MMP-2水平中位数差异有统计学意义（p = 0.012、p = 0.045、p = 0.021、p = 0.032）。经Er-YAG激光辐照的hESCs中位MMP-2值均较高。48小时时，两组肿瘤坏死因子α中位数水平差异有统计学意义（p = 0.029）， Er-YAG激光组肿瘤坏死因子α中位数水平较低。12小时时，两组间IL-6水平中位数差异有统计学意义（p = 0.011）， Er-YAG激光组IL-6水平更高。第72小时，Er-YAG激光组VEGF-A中位数较低（p = 0.021）。24小时各组间IGFBP-1水平中位数差异有统计学意义，Er-YAG激光组IGFBP-1水平较高（p = 0.041）。结论：我们的研究结果表明，非烧蚀性Er-YAG激光诱导hESCs组织重塑，通过促进Th2细胞活性和抑制Th1细胞活性来调节免疫反应。此外，它对去个体化产生了有利的影响。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Lasers in Surgery and Medicine 医学-外科

CiteScore

5.40

自引率

12.50%

发文量

119

审稿时长

1 months

期刊介绍： Lasers in Surgery and Medicine publishes the highest quality research and clinical manuscripts in areas relating to the use of lasers in medicine and biology. The journal publishes basic and clinical studies on the therapeutic and diagnostic use of lasers in all the surgical and medical specialties. Contributions regarding clinical trials, new therapeutic techniques or instrumentation, laser biophysics and bioengineering, photobiology and photochemistry, outcomes research, cost-effectiveness, and other aspects of biomedicine are welcome. Using a process of rigorous yet rapid review of submitted manuscripts, findings of high scientific and medical interest are published with a minimum delay.