Endophytic fungal community composition and function response to strawberry genotype and disease resistance.

IF 2.3 3区生物学 Q2 MULTIDISCIPLINARY SCIENCES

PeerJ Pub Date : 2025-05-09 eCollection Date: 2025-01-01 DOI:10.7717/peerj.19383

Hongjun Yang, Xu Zhang, Rui Wang, Quanzhi Wang, Yuanhua Wang, Geng Zhang, Pengpeng Sun, Bei Lu, Meiling Wu, Zhiming Yan

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引用次数: 0

Abstract

Background: Utilizating the plant endophytic microbiomes to enhance pathogen resistance in crop production is an emerging alternative method to chemical pesticides. However, research on the composition and role of microbial communities related to perennial fruit plants, such as the strawberry, is still limited.

Methods: We provide a comprehensive description of the composition and diversity of fungal communities in three niches (root, stem, and leaf) of three strawberry cultivars ('White Elves', 'Tokun', and 'Akihime') using internal transcribed spacer (ITS) rRNA amplicon sequencing and isolation culture methods. In addition, we also evaluated the disease tolerance ability of three strawberry cultivars to Colletotrichum gloeosporioides and Alternaria alternata through pathogenicity testing.

Results: 'White Elves' has stronger resistance to Colletotrichum gloeosporioides, and Alternaria alternata, followed by 'Tokun', while 'Akihime' has relatively weaker resistance to these pathogens. A total of 258 fungal strains were isolated from healthy strawberry plants and assigned to 34 fungal genera based on morphological and molecular characteristics analysis. Beneficial fungal genera such as Trichoderma and Talaromyces were more prevalent in 'White Elves', whereas common pathogenic fungi in strawberry, such as Colletotrichum, Alternaria, and Fusarium, were more prevalent in 'Akihime'. The composition and diversity of microbial communities vary among genotypes, and resistance to pathogens may play dominant roles in determining the microbial community structure. This study's results aid the biological control of strawberry fungal diseases and are useful for plant microbiome engineering in strawberry cultivation.

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草莓内生真菌群落组成及功能对基因型和抗病性的响应

背景：利用植物内生微生物群增强作物抗病性是一种新兴的替代化学农药的方法。然而，对多年生水果植物（如草莓）中微生物群落的组成和作用的研究仍然有限。方法：采用ITS rRNA扩增子测序和分离培养方法，对3个草莓品种（‘White Elves’、‘Tokun’和‘Akihime’）的根、茎、叶3个生态位（根、茎、叶）真菌群落的组成和多样性进行了全面描述。此外，我们还通过致病性试验，评价了3个草莓品种对炭疽病菌（Colletotrichum gloeosporioides）和交替病菌（Alternaria alternata）的抗病能力。结果：“白精灵”对炭疽病菌和交替病菌的抗性最强，“托坤”次之，“明姬”对这些病菌的抗性相对较弱。从健康草莓植株中分离到258株真菌，经形态学和分子特征分析，隶属于34个真菌属。有益真菌属，如木霉和Talaromyces在“白精灵”中更为普遍，而草莓中常见的致病真菌，如炭疽菌、Alternaria和镰刀菌，在“秋叶”中更为普遍。微生物群落的组成和多样性因基因型而异，对病原体的抗性可能是决定微生物群落结构的主要因素。本研究结果有助于草莓真菌病害的生物防治，对草莓栽培中的植物微生物组工程具有指导意义。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

PeerJ MULTIDISCIPLINARY SCIENCES-

CiteScore

4.70

自引率

3.70%

发文量

1665

审稿时长

10 weeks

期刊介绍： PeerJ is an open access peer-reviewed scientific journal covering research in the biological and medical sciences. At PeerJ, authors take out a lifetime publication plan (for as little as $99) which allows them to publish articles in the journal for free, forever. PeerJ has 5 Nobel Prize Winners on the Board; they have won several industry and media awards; and they are widely recognized as being one of the most interesting recent developments in academic publishing.