Metagenomic Insights Into the Subgingival Microbiome in Periodontal Health and Different Grades of Periodontitis.

IF 3.4 3区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of periodontal research Pub Date : 2025-05-09 DOI:10.1111/jre.13408

Yan Zhao, Lu Song, Hao-Yu Li, Shu-Xin Liu, Fei-Fei Mao, Xiao-Jun Li, Pei-Hui Ding

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引用次数: 0

Abstract

Aim: This cross-sectional study compared the subgingival microbiome in periodontal health (PH) and periodontitis, focusing on distinguishing Stage III Grade B periodontitis (PD-S3gB) and Stage III Grade C periodontitis (PD-S3gC) as defined by the 2018 Classification of Periodontitis.

Methods: Subgingival samples from subjects with PH, PD-S3gB, and PD-S3gC were analyzed using metagenomic sequencing. Taxonomic and functional annotations were performed, followed by analyses of microbial diversity, differential abundance, interspecies networks, predictive modeling, and functional pathway enrichment.

Results: Significant differences in both alpha and beta diversity were observed between PH and periodontitis. Several periodontal pathogens were more abundant in disease states, with Capnocytophaga granulosa and Capnocytophaga sp. CM59 enriched in PD-S3gC compared to PD-S3gB. The PD-S3gC group also exhibited a more complex microbial network with increased interspecies connectivity. An 11-species diagnostic model effectively distinguished PH, PD-S3gB, and PD-S3gC. Furthermore, pathways related to motility, chemotaxis, and methane metabolism were significantly enriched in periodontitis.

Conclusion: Distinct structural and functional differences in the subgingival microbiome characterize periodontal health and periodontitis. Periodontitis with a rapid rate of progression is marked by specific pathogen overgrowth and enhanced microbial interactions, supporting the development of microbiome-based diagnostics and personalized therapies.

Trial registration: Chinese Clinical Trial Registration: ChiCTR2000039426.

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牙周健康和不同等级牙周炎中龈下微生物组的宏基因组学研究。

目的：本横断面研究比较牙周健康（PH）和牙周炎的龈下微生物组，重点区分2018年牙周炎分类中定义的III期B级牙周炎（PD-S3gB）和III期C级牙周炎（PD-S3gC）。方法：采用宏基因组测序对PH、PD-S3gB和PD-S3gC受试者的龈下样本进行分析。进行了分类和功能注释，随后进行了微生物多样性、差异丰度、种间网络、预测建模和功能途径富集分析。结果：在酸碱度和牙周炎之间观察到α和β多样性的显著差异。几种牙周病原体在疾病状态下更为丰富，与PD-S3gC相比，PD-S3gC中富含Capnocytophaga granusa和Capnocytophaga sp. CM59。PD-S3gC组还表现出更复杂的微生物网络，物种间连通性增加。11种诊断模型有效区分了PH、PD-S3gB和PD-S3gC。此外，牙周炎中与运动性、趋化性和甲烷代谢相关的途径显著丰富。结论：牙龈下微生物群的结构和功能差异是牙周健康和牙周炎的特征。牙周炎的发展速度很快，其特点是特定病原体过度生长和微生物相互作用增强，这支持了基于微生物组的诊断和个性化治疗的发展。试验注册：中文临床试验注册：ChiCTR2000039426。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of periodontal research 医学-牙科与口腔外科

CiteScore

6.90

自引率

5.70%

发文量

103

审稿时长

6-12 weeks

期刊介绍： The Journal of Periodontal Research is an international research periodical the purpose of which is to publish original clinical and basic investigations and review articles concerned with every aspect of periodontology and related sciences. Brief communications (1-3 journal pages) are also accepted and a special effort is made to ensure their rapid publication. Reports of scientific meetings in periodontology and related fields are also published. One volume of six issues is published annually.